Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Hi-PurA® Pre filled Cartridges for Tissue RNA Purification
HiPurA® Pre-filled Cartridges for Tissue RNA Purification provides a fast and easy method for purification of total RNA for Northern analysis, Poly A+ RNA selection, Primer extension, RNase and S1 nuclease protection assays, RT-PCR, Differential display, Expression-array and expression-chip analysis and cDNA library construction. The RNA purification procedure using magnetic beads comprises of three steps viz, adsorption of RNA to the magnetic beads, removal of residual contaminants and elution of pure RNA. The RNA obtained is compatible with various downstream applications as mentioned above.
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This kit simplifies isolation of RNA from tissues using magnetic bead based procedure. The RNA – XPress™ Reagent provided in the kit helps in cell disruption and denaturation of tissue material. After adding Chloroform and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing cell debris and DNA and an organic phase containing proteins. The aqueous phase, is then added to the binding solution followed by addition of magnetic beads in Pre?filled cartridge which promotes selective binding of RNA to the magnetic beads. After the initial binding of RNA, impurities like proteins, polysaccharides, low molecular weight metabolites and salts are removed by short washing steps. High quality RNA is finally eluted in the Elution Solution provided with the kit.
| Product Name | Hi-PurA® Pre filled Cartridges for Tissue RNA Purification |
|---|---|
| SKU | MB618PC16200 |
| Customized Product Available | No |
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