Real-Time polymerase chain reaction, also called quantitative Polymerase Chain Reaction (qPCR) or kinetic Polymerase Chain Reaction, is a laboratory technique based on the principle of PCR. This technique is used to amplify a targeted DNA sequence by use of hydrolysis probes that are short oligonucleotides that have a fluorescent reporter dye attached to the 5' end and a quencher dye to the 3' end. HiMedia’s Hi-PCR® HLAB27 Probe PCR Kit is designed to detect HLA-B27 in FAM channel with Endogenous Internal Control in JOE
channel in a single tube reaction.
Human Leukocyte Antigen-B (HLA-B), as part of the major histocompatibility complex (MHC) class I, is
involved in the recognition of self and foreign peptides. The allelic variant of Human Leukocyte Antigen (HLA) serotype B27 (HLA-B27) is present in about 8% of healthy Caucasians and is well known as genetic risk factor for spondyloarthropathies. Up to 96% of patients with ankylosing spondylitis (Morbus Bechterew) and up to 80% of patients with reactive arthritis carry the HLAB27 allele. Ankylosing spondylitis is a chronic, inflammatory arthritis that mainly affects spinal joints and the sacroiliac joint.Real-Time polymerase chain reaction, also called quantitative Polymerase Chain Reaction (qPCR) or kinetic Polymerase Chain Reaction, is a laboratory technique based on the principle of PCR. This technique is used to amplify a targeted DNA sequence by use of hydrolysis probes that are short oligonucleotides that have a fluorescent reporter dye attached to the 5' end and a quencher dye to the 3' end. HiMedia’s Hi-PCR® HLAB27 Probe PCR Kit is designed to detect HLA-B27 in FAM channel with Endogenous Internal Control in JOE channel in a single tube reaction.