Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use:
Recommended for easy and fast detection of urinary tract microorganisms & in disinfectant tesing for determining bactericidal activity.
Composition**
HiCrome™ UTI Agar
| Ingredients | Gms / Litre |
|---|---|
| Peptone, special | 15.000 |
| Chromogenic mixture | 2.450 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Dey Engley Neutralizing Agar
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 10.000 |
| Sodium thiosulphate | 6.000 |
| Sodium thioglycollate | 1.000 |
| Sodium bisulphite | 2.500 |
| Lecithin | 7.000 |
| Polysorbate 80(Tween 80) | 5.000 |
| Bromocresol purple | 0.020 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
-
Surfaces: Loosen cap and remove HiDip™™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface.
Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
-
Liquids: Loosen cap and remove the HiDip™™ slide from container. Check for dehydration or contamination.Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
HiCrome™ UTI Agar is a differential medium recommended for identification and confirmation of microorganisms mainly causing urinary tract infections. It facillitates and expedites the identification of some gram-negative and some gram-positive bacteria on the basis of different contrasted colony colours produced by reactionss of genus or species specific enzymes with two chromogenic substrates to detect presence of β-Glucosidase and β-D-Galactosidase enzyme.
Dey Engley Neutralizing Agar is used in disinfectant testing, where neutralization of the chemical is important for determing its bactericidal activity. It neutralizes a broad spectrum of antiseptics and disinfectants including quatenary ammonium compounds, phenolics, iodine and chlorine preparations, mercurials, formaldehyde and glutraldehyde. Sodium bisulfite neutralizes aldehydes, sodium thioglycollate neutralizes mercurials; sodium thiosulfate neutralizes iodine and chlorine; lecithin neutralizes quaternary ammonium compounds; and polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics. Bromocresol purple is an indicator for dextrose utilization.
Type of specimen
Clinical sample : Urine; Water samples
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
HiCrome™ UTI Agar
- Since it is an enzyme-substrate based reaction, the intensity of colour may vary with isolates.
Dey Engley Neutralizing Agar
- Due to nutritional variations, some strains may show poor growth
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance:
The HiDip™™ slide containing combination of sterile HiCrome™™ UTI Agar and Dey Engley Neutralizing Agar on separate individual surfaces.
Colour:
Colour of HiCrome™ UTI Agar
Light amber coloured medium
Colour of Dey Engley Neutralizing Agar
Reddish purple coloured medium
Quantity of medium:
1.5ml of medium per surface
Sterility test:
Passes release criteria
pH of HiCrome™ UTI Agar:
pH Range: 6.60-7.20
pH of Dey Engley Neutralizing Agar:
pH Range: 7.40-7.80
Cultural Response
Cultural characteristics observed after an incubat ion at 35-37° C for 24-48 hours.
| Organism | Growth | Colour of Colony |
|---|---|---|
| HiCrome™ UTI Agar | ||
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | blue-green (small) |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | blue to purple, mucoid |
| Proteus mirabilis ATCC 12453 | luxuriant | light brown |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | colourless (greenish pigment may be observed) |
| Dey Engley Neutralizing Agar | ||
| Escherichia coli ATCC 25922 (00013*) | luxuriant | |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | |
| Bacillus subtilis subsp. spizizenniii ATCC 6633 (00003*) | luxuriant | |
| Candida albicans ATCC 10231 (00054*) | luxuriant | |
| Aspergillus brasiliensis ATCC 16404 (00053*) | luxuriant | |
Key :- (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
Used HiDip™™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ HiCrome™ UTI Agar - Dey Engley Neutralizing Agar |
|---|---|
| SKU | HD042 |
| Customized Product Available | No |
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