Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for differential & presumptive identification of microorganisms and total bacteria count.
Composition
| HiCrome™ Universal Differential Medium | |
|---|---|
| Peptone | 15.000 Gms / Litre |
| Chromogenic mixture | 2.500 Gms / Litre |
| Tryptone | 4.000 Gms / Litre |
| Agar | 13.500 Gms / Litre |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
| Plate Count Agar | |
|---|---|
| Tryptone | 5.000 Gms / Litre |
| Yeast extract | 2.500 Gms / Litre |
| Dextrose (Glucose) | 1.000 Gms / Litre |
| Agar | 15.000 Gms / Litre |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface.
Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature. - Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination.Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
HiCrome™ Universal Differential Medium is a differential medium recommended for presumptive identification of microorganisms from clinical and non-clinical specimens. These colours are formed due to the reactions of genus or species specific enzymes with the two chromogenic substrates incorporated in the medium. Enterococcus species, E.coli and coliforms produce enzymes which specifically cleave these chromogenic substrates to give characteristically distinctive colony colours. One of the chromogenic substrate is cleaved by β-glucosidase enzyme possessed by Enterococci resulting in the formation of bluish green colonies. E. coli possesses the enzymes β-galactosidase which specifically cleaves the other chromogenic substrate resulting in the formation of purple coloured colonies. E.coli can be differentiated and confirmed from other similar coloured colonies, by performing the indole test.
Plate Count Agar is recommended for the plate count of microorganisms in foods, water and waste water. It is also recommended by APHA, FDA and also by ISO Committee.
Type of specimen
Clinical sample : faecal dilutions; Water samples
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/ eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
HiCrome™ Universal Differential Medium
- Since it is an enzyme-substrate based reaction, the intensity of colour may vary with isolates.
Plate Count Agar
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: The HiDip™ slide containing combination of sterile HiCrome™ Universal Differential Medium and Plate Count Agar Medium on separate individual surfaces.
Colour
Colour of HiCrome™ Universal Differential Medium
Light amber coloured medium
Colour of Plate Count Agar
Light yellow coloured medium
Quantity of medium
1.5ml of medium per surface
Sterility test:
Passes release criteria
pH of HiCrome™ Universal Differential Medium
pH Range: 7.00-7.40
pH of Plate Count Agar
pH Range: 6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37° C for 24-72 hours.
| Organism | Growth | Colour of Colony |
|---|---|---|
| HiCrome™ Universal Differential Medium | ||
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | blue, small |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | purple |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | blue -green, mucoid |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | colourless (greenish pigment may be observed) |
| Proteus mirabilis ATCC 12453 | luxuriant | light brown |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | golden yellow |
| Salmonella Typhi ATCC 6539 | luxuriant | colourless |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | colourless |
| PCA Medium | ||
| Bacillus subtilis subsp. spizizenni ATCC 6633 (00003*) | luxuriant | Red to Maroon |
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | Red to Maroon |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | Red to Maroon |
| Lactobacillus casei ATCC 9595 | luxuriant | Red to Maroon |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | Red to Maroon |
| Streptococcus pyogenes ATCC 19615 | luxuriant | Red to Maroon |
Key
Key :- (*) Corresponding WDCM numbers
Storage and Shelf Life
On receipt store between 2-8°C Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ HiCrome™ Universal Agar-PCA |
|---|---|
| SKU | HD041 |
| Customized Product Available | No |
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