HiDip™ TSA -R2A Agar

Directions

Surfaces: Loosen cap and remove the HiDip slide from container. Aseptically remove the protective plastic cover from slide by taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slide and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for few seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temperature.

Liquids: Loosen cap and remove the HiDip slide from the container. Aseptically remove the protective plastic cover from slide by taking care not to touch agar surfaces. Check for dehydration or contamination. Dip slide into test fluid so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain.

Return the slide to the container and close tightly. Incubate in an upright position at indicated temperature. Label the container for sample number, source, date and time etc. for reference.

Disposal: Used HiDip slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap. An autoclave is not essential, a domestic pressure cooker will suffice.