Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for total bacterial count and isolation of yeast & moulds.
Composition
Plate Count Agar
| Ingredients | g/L |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 1.000 |
| Agar | 15.000 |
| Growth Indicator | 0.100 |
Final pH (at 25°C): 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Malt Extract Agar
| Ingredients | g/L |
|---|---|
| Malt extract | 30.000 |
| Mycological peptone | 5.000 |
| Agar | 15.000 |
Final pH (at 25°C): 5.4±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temperature.
- Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination. Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temperature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
Plate Count Agar:
Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Tryptone provides nitrogenous and carbonaceous compounds, long chain amino acids, and other essential nutrients. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate technique. The samples are diluted and appropriate dilutions are added in Petri plates. Sterile molten agar is added to these plates and plates are rotated gently to ensure uniform mixing of the sample with agar. The poured plate count method is preferred to the surface inoculation method, since it gives higher results. Plate Count Agar is also suitable for enumerating bacterial count of sterile rooms.
Malt extract Agar:
The laboratory diagnosis of fungal infection relies largely on direct as opposed to indirect methods. The use of malt and malt extracts for the propagation of yeasts and moulds is quite common. Reddish (6) described a culture medium prepared from malt extract that was a satisfactory substitute for wort. Malt Extract Medium is similar to the formula of Galloway and Burgess (7) used for the detection, isolation and enumeration of yeasts and moulds. Malt extract provides an acidic environment and nutrients favorable for growth and metabolism of yeasts and moulds. Mycological peptone rapidly gives a luxuriant growth with typical morphology and pigmentation.
Type of specimen
Food and dairy samples; Water samples
Specimen Collection and Handling:
Refer Directions.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
Plate Count Agar/ Malt extract agar
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
- Further biochemical tests must be carried out for further identification
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
The HiDip™ slide containing a combination of sterile PCA Medium and Malt Extract Agar on separate individual surfaces.
Colour
Colour of Plate Count Agar
Light yellow coloured medium
Colour of Malt Extract Agar
Light brown coloured medium
Quantity of medium
2.5 ml per surface
pH of Plate Count Agar
pH Range: 6.90-7.30
pH of Malt Extract Agar
pH Range: 5.20-5.60
Sterility Check :
Passes release criteria
Cultural Response
Cultural characteristics observed after incubation at 35-37°C for 18-24 hours for PCA & at 25 -30°C for 48 - 72 hours for Malt extract agar.
| Organism | Growth | Colour of colony |
|---|---|---|
| PCA Medium | ||
| ** Bacillus spizizenii ATCC 6633 (00003*) | luxuriant | Red to maroon |
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | Red to maroon |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | Red to maroon |
| Lactobacillus casei ATCC 9595 | luxuriant | Red to maroon |
| $Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | Red to maroon |
| Malt Extract Agar | ||
| # Aspergillus brasiliensis ATCC 16404 | luxuriant | - |
| Candida albicans ATCC 10231 (00054*) | luxuriant | Off-white to cream |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | luxuriant | Off-white to cream |
Key :-
- * Corresponding WDCM numbers,
- **Formerly known as Bacillus subtilis subsp. spizizenii
- $Formerly known as Enterobacter aerogenes
- # Formerly known as Aspergillus niger
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (8,9).
| Product Name | HiDip™ PCA - Malt Extract Agar Medium |
|---|---|
| SKU | HD019 |
| Customized Product Available | No |
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