Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for microbial screening of food and water.
Composition
| Ingredients | g / L |
|---|---|
| Plate Count Agar | |
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 1.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
| Violet Red Bile Agar | |
| Peptone | 7.000 |
| Yeast extract | 3.000 |
| Sodium chloride | 5.000 |
| Bile salts mixture | 1.500 |
| Lactose | 10.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.002 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.4±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
- Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination. Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
Plate Count Agar is recommended for the plate count of microorganisms in foods, water and waste water. It is also recommended by APHA, FDA and also by ISO Committee.
VRBA Agar is selective medium used for the detection and enumeration of coliform organisms from water and food products. It is also suggested by ISO Committee for the enumeration of coliforms. It is selective due to presence of the inhibitors-bile salt and crystal violet. Organisms which rapidly ferment lactose produce red colonies surrounded by red-purple halo. Lactose non-fermenters and late lactose fermenters produce pale colonies.
Type of specimen
Food samples; Water samples
Specimen Collection and Handling:
Refer Directions.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations:
Plate Count Agar
- This medium is general purpose medium and may not support the growth of fastidious organisms.
Violet Red Bile Agar
- Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
The HiDip™ slide containing a combination of sterile PCA and VRBA Medium on separate individual surfaces.
Colour
Colour of Plate Count Agar
Light yellow coloured medium
Colour of VRBA medium
Reddish purple coloured medium
Quantity of medium
2.5 ml of medium per surface
Sterility Check
Passes release criteria
pH of Plate Count Agar
pH Range: 6.80-7.20
pH of VRBA medium
pH Range : 7.20-7.60
Cultural Response
Cultural characteristics was observed after incubation at 35-37°C for of 18-24 hours.
| Organism | Growth | Colour of colony |
|---|---|---|
| PCA Medium | ||
| ** Bacillus spizizenii ATCC 6633 (00003*) | luxuriant | Red to Maroon |
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | Red to Maroon |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | Red to Maroon |
| Lactobacillus casei ATCC 9595 | luxuriant | Red to Maroon |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | Red to Maroon |
| Streptococcus pyogenes ATCC 19615 | luxuriant | Red to Maroon |
| VRBA medium | ||
| # Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | pink to pinkish red |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | pinkish red with bile precipitate |
| Salmonella Enteritidis ATCC 13076 (00030*) | luxuriant | colourless to orangish yellow |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited | - |
Key: (*) Corresponding WDCM numbers,
(#) Formerly known as Enterobacter aerogenes
**Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ PCA-VRBA Medium |
|---|---|
| SKU | HD009 |
| Customized Product Available | No |
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