Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for microbial screening of food and water.
Composition
| Ingredients | g / L |
|---|---|
| TSA Agar | |
| Tryptone # | 15.000 |
| Soya peptone | 5.000 |
| Sodium chloride | 5.000 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
| Rose Bengal Chloramphenicol Agar | |
| Mycological peptone | 5.000 |
| Dextrose (Glucose) | 10.000 |
| Potassium dihydrogen phosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Rose bengal | 0.050 |
| Chloramphenicol | 0.100 |
| Agar | 15.500 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Pancreatic digest of casein
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
- Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination. Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
Tryptone Soya Agar is recommended for cultivation of a wide variety of organisms. This medium is in accordance with USP.
Rose Bengal Agar is recommended for the selective isolation and enumeration of yeasts and moulds from environmental materials and food stuffs. Rose bengal is taken up by mould and yeast colonies thereby assist in enumeration. Growth indicator aids in easier observation and enumeration of colonies.
Type of specimen
Food samples, Water samples
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Tryptone Soya Agar: This medium is general purpose medium and may not support the growth of fastidious organisms.
- Rose Bengal Chloramphenicol Agar: The medium should not be exposed to light, since photodegradation of rose bengal yields compounds that are toxic to fungi.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: The HiDip™ slide containing a combination of sterile TSA Medium and Rose Bengal Medium on separate individual surfaces.
Colour of TSA medium: Light yellow coloured medium
Colour of Rose Bengal medium: Deep pink coloured medium
Quantity of medium: 2.5 ml of medium per surface
pH of TSA medium: pH Range : 7.10-7.5
pH of Rose Bengal Medium: pH Range: 7.00-7.40
Sterility Check: Passes release criteria
Cultural Response: Cultural characteristics observed after incubation at 35 - 37°C on TSA Medium for 18-24 hours and at 20 - 25°C on Rose Bengal Medium for 48 - 72 hours.
| Organism | Growth | Colour of the colony |
|---|---|---|
| TSA medium | ||
| ** Bacillus spizizenii ATCC 6633 (00003*) | luxuriant | Red to Maroon |
| ## Phocaeicola vulgatus ATCC 8482 | luxuriant | Red to Maroon |
| Candida albicans ATCC 10231 (00054*) | luxuriant | Red to Maroon |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | Red to Maroon |
| Neisseria meningitidis ATCC 13090 | luxuriant | Red to Maroon |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | Red to Maroon |
| Streptococcus pyogenes ATCC 19615 | luxuriant | Red to Maroon |
| Rose Bengal Medium | ||
| Candida albicans ATCC 10231 (00054*) | Good | Cream to off white |
| Escherichia coli ATCC 25922 (00013*) | inhibited | - |
| # Aspergillus brasiliensis ATCC 16404 | Good | - |
| $ Kokuria rhizophila ATCC 9341 | inhibited | Cream to off white |
| Saccharomyces cerevisiae ATCC 9763 (00058*) | Good | - |
Key: *Corresponding WDCM numbers.
**Formerly known as Bacillus subtilis subsp. spizizenii
$Formerly known as Micrococcus luteus
#Formerly known as Aspergillus niger
##Formerly known as Bacteroides vulgatus
Storage and Shelf Life
Store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ TSA-Rose Bengal Medium |
|---|---|
| SKU | HD008 |
| Customized Product Available | No |
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