Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for easy and fast detection of urinary tract microorganisms.
Composition
Ingredients
MacConkey Agar
| Ingredients | g/L |
|---|---|
| Gelatin peptone | 17.000 |
| Tryptone | 1.500 |
| Peptone | 1.500 |
| Lactose | 10.000 |
| Bile salts | 1.500 |
| Sodium chloride | 5.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.001 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
HiCrome™ UTI Medium
| Peptone, special | 15.000 |
| Chromogenic mixture | 2.450 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
- Surfaces: Loosen cap and remove HiDip™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
- Liquids: Loosen cap and remove the HiDip™ slide from container. Check for dehydration or contamination. Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
MacConkey Agar is a differential medium for the selection and recovery of the Enterobacteriaceae and related enteric gram-negative bacilli. This medium is prepared in accordance with USP and contains crystal violet, Nacl and bile salts. It is very selective and suppresses growth of a number of gram-positive bacteria including Staphylococci.
HiCrome™ UTI Agar is a differential medium recommended for identification and confirmation of microorganisms mainly causing urinary tract infections. It facilitates and expedites the identification of some gram-negative and some gram-positive bacteria on the basis of different contrasted colony colours produced by reactions of genus or species specific enzymes with two chromogenic substrates to detect presence of ß-Glucosidase and ß-D-Galactosidase enzyme.
Type of specimen
Clinical samples: Urine, Food and dairy samples, Water samples.
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
MacConkey Agar:
- Though the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for further confirmation.
HiCrome™ UTI Medium:
- Since it is an enzyme-substrate based reaction, the intensity of colour may vary with isolates.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
The HiDip™ slide contains a combination of sterile MacConkey Agar Medium and HiCrome™ UTI Medium on separate individual surfaces.
Colour
Colour of MacConkey Agar
Red coloured gel with purplish tinge
Colour of HiCrome™ UTI Medium
Light amber coloured medium
Quantity of medium
2.5ml of medium per surface
Sterility Check :
Passes release criteria
pH of MacConkey Agar
pH Range: 6.90-7.30
pH of HiCrome™ UTI Medium
pH Range: 6.60-7.0
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
| Organism | Growth | Colour of colony |
|---|---|---|
MacConkey Agar |
||
| Escherichia coli ATCC 25922 (00013*) | luxuriant | pink to red with bile precipitate |
| # Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | pink to red |
| Enterococcus faecalis ATCC 29212 (00087*) | fair to good | Pale pink to red |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | inhibited | - |
| Salmonella Enteritidis ATCC 13076 (00030*) | luxuriant | colourless |
| Salmonella Paratyphi В ATCC 8759 | luxuriant | colourless |
| Salmonella Typhi ATCC 6539 | luxuriant | colourless |
| ## Proteus hauseri ATCC 13315 | luxuriant | colourless |
| Shigella flexneri ATCC 12022 (00126*) | fair to good | colourless |
HiCrome™ UTI Medium |
||
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | blue, small |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | pink-purple |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | blue to purple, mucoid |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | colourless ( greenish pigment may be observed) |
| Proteus mirabilis ATCC 12453 | luxuriant | light brown |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | golden yellow |
Key :- * Corresponding WDCM numbers
## Formerly known as Proteus vulgaris
# Formerly known as Enterobacter aerogenes
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ Mac-HiCrome™ UTI Medium |
|---|---|
| SKU | HD007 |
| Customized Product Available | No |
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