Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for easy and fast detection of urinary tract microorganisms.
Composition
| Ingredients | g/L |
|---|---|
| C.L.E.D. Agar w/Bromo Thymol Blue | |
| Peptone | 4.000 |
| Tryptone | 4.000 |
| HM Peptone B# | 3.000 |
| Lactose | 10.000 |
| L-Cystine | 0.128 |
| Bromothymol blue | 0.020 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
| HiCrome™ UTI Medium | |
| Peptone, special | 15.000 |
| Chromogenic mixture | 2.450 |
| Agar | 15.000 |
| Final pH (at 25°C) | 6.8±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Directions
1. Surfaces: Loosen cap and remove HiDip™™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temperature.
2. Liquids: Loosen cap and remove the HiDip™™ slide from container. Check for dehydration or contamination.Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
C.L.E.D.Agar is recommended for isolation, enumeration and identification of urinary pathogens on the basis of lactose fermentation.
HiCrome™ UTI Agar is a differential medium recommended for identification and confirmation of microorganisms mainly causing urinary tract infections. It facilitates and expedites the identification of some gram-negative and some gram-positive bacteria on the basis of different contrasted colony colours produced by reactions of genus or species specific enzymes with two chromogenic substrates to detect presence of β-Glucosidase and β-D-Galactosidase enzyme.
Type of specimen
Clinical samples : Urine; Food and dairy samples, Water samples.
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
C.L.E.D.Agar
- 1. This medium is recommended for urine infection. Low urine count may be a result of antibiotic therapy, low pH of urine.
- 2. Recovery depends on the urine count.
- 3. Inoculate the medium immediately after urine collection.
HiCrome™ UTI Medium
- 1. Since it is an enzyme-substrate based reaction, the intensity of colour may vary with isolates.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: The HiDipTMslide contains a combination of sterile CLED Agar Medium and HiCrome™м UTI Medium on separate individual surfaces.
Colour of CLED Agar: Green coloured medium
Colour of HiCrome™ UTI Medium: Light amber coloured medium
Quantity of medium: 2.5ml per surface
Sterility Check: Passes release criteria
pH of CLED Agar: pH Range : 7.20-7.60
pH of HiCrome™ UTI Medium: pH Range: 6.60-7.0
Cultural Response: Cultural characteristics observed after an incubation at 35-37° C for 18-24 hours.
| Organism | Growth | Colour of colony |
|---|---|---|
| CLED Agar | ||
| Escherichia coli ATCC 25922 (00013*) | good-luxuriant | yellow, opaque, centre slightly deeper yellow |
| Enterococcus faecalis ATCC 29212 (00087*) | good-luxuriant | slight yellowish or greenish |
| ## Proteus hauseri ATCC 13315 | good-luxuriant | blue |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | good-luxuriant | deep yellow |
| Salmonella Typhi ATCC 6539 | good-luxuriant | bluish |
| HiCrome™ UTI Medium | ||
| Enterococcus faecalis ATCC 29212 (00087*) | luxuriant | blue, small |
| Escherichia coli ATCC 25922 (00013*) | luxuriant | pink-purple |
| Klebsiella pneumoniae ATCC 13883 (00097*) | luxuriant | blue to purple, mucoid |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | colourless (greenish pigment may be observed) |
| Proteus mirabilis ATCC 12453 | luxuriant | light brown |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | luxuriant | golden yellow |
Key :- * Corresponding WDCM numbers ## Formerly known as Proteus vulgaris
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ CLED-HiCrome™ UTI Medium |
|---|---|
| SKU | HD006 |
| Customized Product Available | No |
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