Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Recommended for urine bacteria screening.
Composition
C.L.E.D. Agar w/Bromo Thymol Blue
| Ingredients | g/L |
|---|---|
| Peptone | 4.000 |
| Tryptone | 4.000 |
| HM Peptone B# | 3.000 |
| Lactose | 10.000 |
| L-Cystine | 0.128 |
| Bromothymol blue | 0.020 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.3±0.2 |
**Formula adjusted, standardized to suit performance parameters
# - Equivalent to Beef extract
Cetrimide Agar Base
| Ingredients | g/L |
|---|---|
| Gelatin peptone | 20.000 |
| Magnesium chloride | 1.400 |
| Potassium sulphate | 10.000 |
| Cetrimide | 0.300 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
MacConkey Agar w/ 0.15% Bile Salts, CV and NaCl
| Ingredients | g/L |
|---|---|
| Gelatin peptone | 17.000 |
| Tryptone | 1.500 |
| Peptone | 1.500 |
| Lactose | 10.000 |
| Bile salts | 1.500 |
| Sodium chloride | 5.000 |
| Neutral red | 0.030 |
| Crystal violet | 0.001 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.1±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
- Surfaces: Loosen cap and remove HiDip™™ slide from container taking care not to touch agar surfaces. Check for dehydration or contamination. Gently lower the slides and press agar to touch the test surface by bending the scull around the hinge line. Apply even and firm pressure for 15-20 seconds. Take care not to smudge agar over the test surface. Repeat procedure using the second agar surface on an area adjacent to the initial test side. Return the slide to the container and close tightly. Incubate in an up right position at indicated temprature.
- Liquids: Loosen cap and remove the HiDip™™ slide from container. Check for dehydration or contamination. Dip slide into test fluid for upto 15-20 seconds so that agar surface becomes totally covered. (In case of inadequate liquid sample availability, pour sample over the surface of the slide). Allow to drain. Tab it gently to remove excess fluid from surface. Return the slide to the container and close tightly. Incubate in an upright position at indicated temprature. Label the container for sample number, source, date and time etc. for reference.
Principle And Interpretation
C.L.E.D.Agar is recommended for isolation, enumeration and identification of urinary pathogens on the basis of lactose fermentation.
Cetrimide Agar Base is used for the selective isolation of Pseudomonas aeruginosa from clinical specimens. It is a selective media that contains cetrimide inhibits the growth of many microorganisms whilst allowing Pseudomonas aeruginosa to develop typical colonies
MacConkey Agar is a differential medium for the selection and recovery of the Enterobacteriaceae and related enteric gram-negative bacilli. This medium is prepared in accordance with USP and contains crystal violet, Nacl and bile salts. It is very selective and suppresses growth of a number of gram-positive bacteria including Staphylococci.
Type of Specimen
Clinical sample: Urine; Food and dairy samples, Water samples.
Specimen Collection and Handling
Refer Directions.
Warning and Precautions
In Vitro diagnostic use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
C.L.E.D.Agar
- This medium is recommended for urine infection. Low urine count may be a result of antibiotic therapy, low pH of urine.
- Recovery depends on the urine count.
- Inoculate the medium immediately after urine collection.
Cetrimide Agar Base:
- This medium is a selective medium, some strains may show poor growth as cetrimide is highly toxic.
- Further biochemical tests must be carried out for further confirmation.
MacConkey Agar:
- Though the medium is recommended for selective isolation, further biochemical and serological testing must be carried out for further confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
The HiDip™™ slide containing combination of sterile CLED Medium, Cetrimide Agar and MacConkey Agar on separate individual surfaces.
Colour
Colour of CLED Agar
Green coloured gel.
Colour of Cetrimide Agar
Light amber coloured gel
Colour of MacConkey Agar
Red coloured gel with puplish tinge
Quantity of medium
1.5ml of medium per surface
Sterility Check :
Passes release criteria
pH of CLED Agar
pH Range: 7.10-7.50
pH of Cetrimide Agar
pH Range: 7.00-7.40
pH of MacConkey Agar
pH Range: 6.90 - 7.30
Cultural Response
Cultural characteristics observed after an incubation at 35-37° C for 18-48 hours.
| Organism | Growth | Colour of colony | Pigment |
|---|---|---|---|
| CLED Agar | |||
| Escherichia coli ATCC 25922 (00013*) | good-luxuriant | yellow, opaque, centre slightly deeper yellow | - |
| Enterococcus faecalis ATCC 29212 (00087*) | good-luxuriant | slight yellowish or greenish | - |
| ## Proteus hauseri ATCC 13315 | good-luxuriant | blue | - |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | good-luxuriant | deep yellow | - |
| Salmonella Typhi ATCC 6539 | good-luxuriant | bluish | - |
| Cetrimide Agar | |||
| ^Pseudomonas paraeruginosa ATCC 9027 (00026*) | luxuriant | - | yellow green |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | luxuriant | - | yellow green |
| Stenotrophomonas maltophila ATCC 13637 | inhibited | - | - |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | inhibited | - | - |
| Escherichia coli ATCC 25922 (00013*) | inhibited | - | - |
| MacConkey Agar | |||
| Escherichia coli ATCC 25922 (00013*) | luxuriant | pink to red with bile precipitate | - |
| # Klebsiella aerogenes ATCC 13048 (00175*) | luxuriant | pink to red | - |
| Enterococcus faecalis ATCC 29212 (00087*) | fair to good | colourless to pale pink | - |
| Salmonella Typhimurium ATCC 14028 (00031*) | luxuriant | colourless | - |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | inhibited | - | - |
| Salmonella Enteritidis ATCC 13076 (00030*) | luxuriant | colourless | - |
| Salmonella Paratyphi A ATCC 9150 | luxuriant | colourless | - |
| Salmonella Paratyphi B ATCC 8759 | luxuriant | colourless | - |
| Salmonella Typhi ATCC 6539 | luxuriant | colourless | - |
| ## Proteus hauseri ATCC 13315 | luxuriant | colourless | - |
| Shigella flexneri ATCC 12022 (00126*) | fair to good | colourless | - |
Key :- * Corresponding WDCM numbers
^ Formerly known as Pseudomonas aeruginosa
# Formerly known as Enterobacter aerogenes
## Formerly known as Proteus vulgaris
Storage and Shelf Life
On receipt store between 15-30°C. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
Used HiDip™™ slides should be handled carefully, as it contains live microorganisms. These slides can be best disposed off either by or by immersing in a suitable disinfectant solution (i.e. dettol, phenyl etc.) over night or by autoclaving them after loosening the cap.
| Product Name | HiDip™ CLED-Cetri-Mac Medium |
|---|---|
| SKU | HD001 |
| Customized Product Available | No |
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