Asparagine Proline Broth, Granulated

Principle And Interpretation

Pseudomonas aeruginosa is one of the major contaminants of natural, fresh and recreational water, with the entry being contaminated by wastewater. Ps. aeruginosa is an opportunistic pathogen that can multiply in recreational waters in the presence of sufficient nutrients. It produces a water soluble, fluorescent pigment in media containing asparagine and ethanol. Asparagine Proline Broth is recommended for cultivation of Ps. aeruginosa by the membrane filter technique.in accordance with BIS standard (1).

Asparagine Proline Broth contains both the enantiomeric forms of Aspargine, which is readily utilized by Pseudomonas for their growth. Phosphate and sulphates provide the ions for the growth as well as buffers the medium to promote the growth of the organism.

When 1 ml of sample is to be analyzed, add 1 ml of sample to 4 ml of single strength medium (14.5 g/l). If larger portions of the sample (10 ml, 50 ml) are to be used, add the sample to an equal volume of the concentrated medium (23.2 g/l). Incubate at 37 ±1°C for 48 hours. Examine for growth and fluorescence. The growth is further sub cultured on Milk Agar w/ Cetrimide (M1273S).