Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Synthetic Complete Supplement Mixture (SC) w/o LEU
Synthetic Complete Supplement Mixture for amino acids to prepare appropiate media for selection of LEU2 auxotrophic strains and transformants
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Composition** :
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 21.00 |
| L-Alanine | 85.60 |
| L-Arginine HCI | 85.60 |
| L-Asparagine | 85.60 |
| L-Aspartic acid | 85.60 |
| L-Cysteine HCI | 85.60 |
| Glutamine | 85.60 |
| L-Glutamic acid | 85.60 |
| Glycine | 85.60 |
| L-Histidine HCl | 85.60 |
| Myo-Inositol | 85.60 |
| L-Isoleucine | 85.60 |
| L-Lysine HCl | 85.60 |
| L-Methionine | 85.60 |
| L-Phenylalanine | 85.60 |
| L-Proline | 85.60 |
| L-Serine | 85.60 |
| L-Threonine | 85.60 |
| L-Tryptophan | 85.60 |
| L-Tyrosine | 85.60 |
| Uracil | 85.60 |
| L-Valine | 85.60 |
** Formula adjusted, standardized to suit performance parameters
Directions :
Suspend 1.83 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation :
Synthetic Complete Supplement Mixture (SC) w/o LEU is used as a dropout supplement for all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.
Synthetic Complete Supplement Mixture (SC) w/o LEU supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae except leucine. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), Ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of leucine prototrophs and wild type strains of S. cerevisiae but leucine auxotroph cannot grow in this media. Therefore, SC w/o LEU can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.
Quality Control :
Appearance of Powder : White to cream homogeneous, free flowing powder.
Colour and Clarity : Colourless to light yellow coloured, clear solution without any precipitate.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life :
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Synthetic Complete Supplement Mixture (SC) w/o LEU |
|---|---|
| SKU | G153 |
| Customized Product Available | No |
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