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Synthetic Complete Supplement Mixture (SC) w/o HIS
Synthetic Complete Supplement Mixture for amino acids to prepare appropiate media for selection of HIS3 auxotrophic strains and transformants
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G152
Synthetic Complete Supplement Mixture (SC) w/o HIS is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition**
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 21.00 |
| L-Alanine | 85.60 |
| L-Arginine HC1 | 85.60 |
| L-Asparagine | 85.60 |
| L-Aspartic acid | 85.60 |
| L-Cysteine HCI | 85.60 |
| Glutamine | 85.60 |
| L-Glutamic acid | 85.60 |
| Glycine | 85.60 |
| Myo-Inositol | 85.60 |
| L-Isoleucine | 85.60 |
| L-Leucine | 173.40 |
| L-Lysine HCl | 85.60 |
| L-Methionine | 85.60 |
| L-Phenylalanine | 85.60 |
| L-Proline | 85.60 |
| L-Serine | 85.60 |
| L-Threonine | 85.60 |
| L-Tryptophan | 85.60 |
| L-Tyrosine | 85.60 |
| Uracil | 85.60 |
| L-Valine | 85.60 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 1.91 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Synthetic Complete Supplement Mixture (SC) w/o HIS is used as a dropout supplement for all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.
Synthetic Complete Supplement Mixture (SC) w/o HIS supplies the essential nutritional elements for budding yeast cells. It is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae except histidine. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of histidine prototrophs and wild type strains of S. cerevisiae but histidine auxotroph cannot grow in this media. Therefore, SC w/o HIS can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.
Quality Control
Appearance of Powder : White to light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Colourless to pale yellow coloured, clear solution without any precipitate.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Storage temperature: 10°C - 30°C
Do not use if package is damaged
| Product Name | Synthetic Complete Supplement Mixture (SC) w/o HIS |
|---|---|
| SKU | G152 |
| Customized Product Available | No |
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