Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Complete Supplement Mixture w/o TRP-URA
Complete supplement for amino acids to prepare appropiate media for selection of URA3 and TRP1 auxotrophic strains and transformants
Your enquiry has been submitted
Please login to Post an Enquiry.
Complete Supplement Mixture w/o TRP-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HCI | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Histidine HCl | 20.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 100.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tyrosine | 50.00 |
| L-Valine | 140.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 720 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture w/o TRP-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae. Complete Supplement Mixture (CSM) is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. Furthermore, it contains uracil. CSM w/o TRP-URA does not contain tryptophan and uracil. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), Ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of tryptophan and uracil prototrophs and wild type strains of S. cerevisiae but tryptophan or uracil auxotroph cannot grow in this media. Therefore, CSM w/o TRP-URA can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.
Quality Control
Appearance of Powder : White to light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Colourless to creamish yellow coloured, clear solution without any precipitate.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Complete Supplement Mixture w/o TRP-URA |
|---|---|
| SKU | G121 |
| Customized Product Available | No |
Write Your Own Review