Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Complete Supplement Mixture w/o LEU-URA
Complete supplement for amino acids to prepare appropiate media for selection of LEU2 and URA3 auxotrophic strains and transformants
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Complete Supplement Mixture w/o LEU-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HCl | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Histidine HCl | 20.00 |
| L-Isoleucine | 50.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tryptophan | 50.00 |
| L-Tyrosine | 50.00 |
| L-Valine | 140.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 670 mg in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture w/o LEU-URA is used as a dropout supplement for all strains of Saccharomyces cerevisiae. Complete Supplement Mixture (CSM) is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It also contains uracil. CSM w/o LEU-URA does not contain leucine and uracil. This makes it a double dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose). A leucine and uracil auxotrophic yeast mutant strain cannot grow on this media but a wild-type or a leucine and uracil prototrophic yeast strain can grow.
Quality Control
Appearance of Powder :
White to cream coloured, homogeneous, free flowing powder.
Colour and Clarity :
Colourless clear solution without any precipitate.
Cultural Response :
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC9763 | good-luxuriant |
Storage and Shelf-life :
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Complete Supplement Mixture w/o LEU-URA |
|---|---|
| SKU | G117 |
| Customized Product Available | No |
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