Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Complete Supplement Mixture w/o HIS is used as a dropout supplement for all strains of Saccharomyces cerevisiae.
Composition**
| Ingredients | Milligrams/Litre |
|---|---|
| Adenine | 10.00 |
| L-Arginine HCl | 50.00 |
| L-Aspartic acid | 80.00 |
| L-Isoleucine | 50.00 |
| L-Leucine | 100.00 |
| L-Lysine HCl | 50.00 |
| L-Methionine | 20.00 |
| L-Phenylalanine | 50.00 |
| L-Threonine | 100.00 |
| L-Tryptophan | 50.00 |
| L-Tyrosine | 50.00 |
| Uracil | 20.00 |
| L-Valine | 140.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 770 mg in 1000 ml distilled water. Sterilize by autoclaving at 115°C for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Complete Supplement Mixture w/o HIS is used as a dropout supplement for all strains of Saccharomyces cerevisiae. Complete Supplement Mixture (CSM) is composed of all the amino acids required for the vigorous growth of Saccharomyces cerevisiae. It also contains uracil. CSM w/o HIS does not contain histidine. This makes it a dropout growth medium for yeast cells. It can be mixed with yeast nitrogen base (YNB), ammonium sulphate and an appropriate carbon source (glucose/galactose/raffinose) to produce a media suitable for the growth of histidine prototrophs and wild type strains of S. cerevisiae but histidine auxotroph cannot grow in this media. Therefore, CSM w/o HIS can be used as drop-out formulation for the selection of auxotrophic requirements and transformants.
Quality Control
Appearance of Powder: White to off-white coloured, homogeneous, free flowing powder.
Colour and Clarity of prepared medium: Colourless, clear solution without any precipitate.
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Storage temperature for powder: 10°C - 30°C
Do not use if package is damaged.
Expiry: 05/2025
| Product Name | Complete Supplement Mixture w/o HIS |
|---|---|
| SKU | G105 |
| Customized Product Available | No |
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