Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Yeast Nitrogen Base (YNB) w/o Ammonium sulphate, w/o Potassium phosphate
Yeast nitrogen base for selecting yeast strains based on carbon and nitrogen requirements
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Intended Use
Yeast Nitrogen Base (YNB) w/o Ammonium Sulphate, w/o Potassium Phosphate is used for the growth of all strains of Saccharomyces cerevisiae.
Composition
| Ingredients | Milligrams/Litre |
|---|---|
| Magnesium sulphate | 500.00 |
| Sodium chloride | 100.00 |
| Calcium chloride | 100.00 |
| Biotin | 0.002 |
| Calcium pantothenate | 0.40 |
| Folic acid | 0.002 |
| Inositol | 2.00 |
| Niacin | 0.40 |
| PABA | 0.20 |
| Pyridoxin, HCI | 0.40 |
| Riboflavin | 0.20 |
| Thiamine HCl | 0.40 |
| Boric acid | 0.50 |
| Copper sulphate | 0.04 |
| Potassium iodide | 0.10 |
| Ferric chloride | 0.20 |
| Manganese sulphate | 0.40 |
| Sodium molybdate | 0.20 |
| Zinc sulphate | 0.40 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 0.7 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
Yeast Nitrogen Base (YNB) w/o Ammonium Sulphate w/o Potassium Phosphate is used for the growth of all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology. Yeast Nitrogen Base (YNB) w/o Ammonium Sulfate w/o Potassium Phosphate is developed according to the formulae of Wickerham (1) and Burkholder (2) which supplies the required nutritional elements for budding yeast cells. This media is used for the classification of yeast strains based on the carbon and nitrogen requirements and it contains all the essential vitamins and inorganic salts except Ammonium sulphate and Potassium phosphate which are required for the propagation of yeast cells. Furthermore, this media does not contain the amino acids namely, histidine, methionine, leucine, lysine and tryptophan.
Quality Control
Appearance of Powder: White to cream coloured, homogeneous, free flowing powder.
Colour and Clarity: Colourless, clear solution without any precipitate.
Cultural Response: Cultural characteristics observed after incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Yeast Nitrogen Base (YNB) w/o Ammonium sulphate, w/o Potassium phosphate |
|---|---|
| SKU | G095 |
| Customized Product Available | No |
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