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Yeast Nitrogen Base (YNB) w/ Ammonium sulphate, w/o Phosphates, w/o Sodium chloride

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SKU:
G094
Yeast nitrogen base for selecting yeast strains based on carbon and nitrogen requirements

Yeast Nitrogen Base (YNB) w/ Ammonium Sulphate, w/o Phosphates, w/o Sodium Chloride is used for the growth of all strains of Saccharomyces cerevisiae.

Composition**

Ingredients Milligrams/Litre
Magnesium sulphate 500.00
Calcium chloride 100.00
Biotin 0.002
Calcium pantothenate 0.40
Folic acid 0.002
Inositol 2.00
Niacin 0.40
PABA 0.20
Pyridoxin, HCl 0.40
Riboflavin 0.20
Thiamine HCl 0.40
Boric acid 0.50
Copper sulphate 0.04
Potassium iodide 0.10
Ferric chloride 0.20
Manganese sulphate 0.40
Sodium molybdate 0.20
Zinc sulphate 0.40
Ammonium sulphate 5000.00

** Formula adjusted, standardized to suit performance parameters

Directions

Suspend 5.6 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.

Principle and Interpretation

Yeast Nitrogen Base (YNB) w/ Ammonium Sulphate, w/o Phosphates, w/o Sodium Chloride is used for the growth of all strains of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.

Yeast Nitrogen Base (YNB) w/ Ammonium Sulfate w/o Phosphates, w/o Sodium Chloride is developed according to the formulae of Wickerham (1) and Burkholder (2) which supplies the required nutritional elements for budding yeast cells. This media is used for the classification of yeast strains based on the carbon and nitrogen requirements and it contains all the essential vitamins and inorganic salts except Potassium phosphate and Sodium chloride which are required for the propagation of yeast cells. Furthermore, this media does not contain the amino acids namely, histidine, methionine, leucine, lysine and tryptophan.

Quality Control

  • Appearance of Powder:

    White to cream coloured, homogeneous, free flowing powder.

  • Colour and Clarity:

    Colourless, clear solution without any precipitate.

  • Cultural Response:

    Cultural characteristics observed after incubation at 25-30°C for 18 - 48 hours.

    Organisms (ATCC) Growth
    Saccharomyces cerevisiae ATCC 9763 good-luxuriant

Storage and Shelf-life

Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.

More Information
Product Name Yeast Nitrogen Base (YNB) w/ Ammonium sulphate, w/o Phosphates, w/o Sodium chloride
SKU G094
Customized Product Available No
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