Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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DOBA Growth Medium with 2% Galactose and 1% Raffinose is used for the growth of Saccharomyces cerevisiae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Potassium dihydrogen phosphate | 1.00 |
| Magnesium sulphate | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride | 0.10 |
| Biotin | 0.002 mg |
| Calcium pantothenate | 0.4 mg |
| Folic acid | 0.002 mg |
| Inositol | 2.00 mg |
| Niacin | 0.4 mg |
| PABA | 0.2 mg |
| Pyridoxin, HCI | 0.4 mg |
| Riboflavin | 0.2 mg |
| Thiamine HCl | 0.4 mg |
| Boric acid | 0.5 mg |
| Copper sulphate | 0.04 mg |
| Potassium iodide | 0.1 mg |
| Ferric chloride | 0.2 mg |
| Manganese sulphate | 0.4 mg |
| Sodium molybdate | 0.2 mg |
| Zinc sulphate | 0.4 mg |
| Ammonium sulphate | 5.00 |
| Galactose | 20.00 |
| Raffinose | 10.00 |
| Agar | 15.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 51.7 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
DOBA Growth Medium with 2% Galactose and 1% Raffinose is used for the growth of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.
DOBA Growth Medium with 2% Galactose and 1% Raffinose is developed according to the formulae of Wickerham (1, 2) which supplies the required nutritional elements for budding yeast cells. This media is used for the classification of yeast strains based on the carbon and nitrogen requirements and it contains all the essential vitamins and inorganic salts except Ammonium sulphate which is required for the propagation of yeast cells. Furthermore, this media does not contain the amino acids namely, histidine, methionine, leucine, lysine and tryptophan. Galactose and raffinose serve as the carbon source for the cells.
Quality Control
Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae | good-luxuriant |
References
- Wickerham. 1939. J. Tropical Med. Hyg. 42:176.
- Wickerham. 1946. J. Bacteriol. 52:293.
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | DOBA Growth Medium with 2% Galactose and 1% Raffinose |
|---|---|
| SKU | G089 |
| Customized Product Available | No |
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