Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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DOBA Growth Medium with 2% Galactose is used for the growth of Saccharomyces cerevisiae.
Composition** :
| Ingredients | Grams/Litre |
|---|---|
| Potassium dihydrogen phosphate | 1.00 |
| Magnesium sulphate | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride | 0.10 |
| Biotin | 0.002 mg |
| Calcium pantothenate | 0.4 mg |
| Folic acid | 0.002 mg |
| Inositol | 2.00 mg |
| Niacin | 0.4 mg |
| PABA | 0.2 mg |
| Pyridoxin, HCl | 0.4 mg |
| Riboflavin | 0.2 mg |
| Thiamine HCl | 0.4 mg |
| Boric acid | 0.5 mg |
| Copper sulphate | 0.04 mg |
| Potassium iodide | 0.1 mg |
| Ferric chloride | 0.2 mg |
| Manganese sulphate | 0.4 mg |
| Sodium molybdate | 0.2 mg |
| Zinc sulphate | 0.4 mg |
| Ammonium sulphate | 5.00 |
| Galactose | 20.00 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions :
Suspend 41.7 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation :
DOBA Growth Medium with 2% Galactose is used for the growth of Saccharomyces cerevisiae.
Quality Control :
Appearance of Powder :
Light yellow coloured, homogeneous, free flowing powder.
Gelling :
Firm, comparable with 1.5% Agar gel.
Colour and Clarity :
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response :
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae | good-luxuriant |
Storage and Shelf-life :
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | DOBA Growth Medium with 2% Galactose (Glucose free) |
|---|---|
| SKU | G085 |
| Customized Product Available | No |
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