Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
DOB Growth Medium is used for the growth of Saccharomyces cerevisiae.
| Ingredients | Grams/Litre |
|---|---|
| Potassium dihyrogen phosphate | 1.00 |
| Magnesium sulphate | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride | 0.10 |
| Biotin | 0.002 mg |
| Calcium pantothenate | 0.4 mg |
| Folic acid | 0.002 mg |
| Inositol | 2.00 mg |
| Niacin | 0.4 mg |
| PABA | 0.2 mg |
| Pyridoxin, HCl | 0.4 mg |
| Riboflavin | 0.2 mg |
| Thiamine HCl | 0.4 mg |
| Boric acid | 0.5 mg |
| Copper sulphate | 0.04 mg |
| Potassium iodide | 0.1 mg |
| Ferric chloride | 0.2 mg |
| Manganese sulphate | 0.4 mg |
| Sodium molybdate | 0.2 mg |
| Zinc sulphate | 0.4 mg |
| Ammonium sulphate | 5.00 |
| Dextrose | 20.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 26.7 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
DOB Growth Medium is developed according to the formulae of Wickerham (1, 2) which supplies the required nutritional elements for budding yeast cells. This media contains all the essential vitamins and inorganic salts which are required for the propagation of yeast cells but does not contain the amino acids namely, histidine, methionine, leucine, lysine and tryptophan. Dextrose serves as the carbon source for the cells. DOB Growth Medium is used for the growth of Saccharomyces cerevisiae. This yeast strain is called budding yeast and is extensively studied microorganism in molecular and cell biology.
Quality Control
Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity: Light yellow coloured, clear solution without any precipitate.
Cultural Response: Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | DOB Growth Medium |
|---|---|
| SKU | G082 |
| Customized Product Available | No |
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