SDA Growth Medium w/o LEU includes a yeast nitrogen base along with ammonium sulfate, and dextrose as the carbon source, which is further supplemented with various amino acids except Leucine which makes it a dropout growth medium for yeast cells allowing only leucine prototrophic yeast strain to grow. It is used for selection of auxotrophic phenotypes in yeast, selection for plasmid after yeast transformation, for yeast one/two hybrid screening. It contains 1.5% agar as solidifying agent