SD Growth Medium w/o LEU-URA

Principle and Interpretation :

SD Growth Medium w/o LEU-URA is a synthetic defined media for the selective growth of Saccharomyces cerevisiae. Synthetically Defined media known as Yeast Nitrogen Base Media for the growth of yeast cells were first cited by Wickerham (1, 2). Synthetic defined Growth Medium w/o LEU-URA includes a yeast nitrogen base along with ammonium sulfate and dextrose as the carbon source, which is further supplemented with various amino acids except leucine and uracil. This makes it a double dropout growth medium for yeast cells. A leucine and uracil auxotrophic yeast mutant strain cannot grow on this media but a wild-type or a leucine and uracil prototrophic yeast strain can grow. The leucine and uracil auxotroph has mutation in the genes (e.g. LEU2 and URA3) of the leucine as well as uracil synthesis pathway and this mutant strain will grow in this medium if leucine and uracil are supplied from outside e.g. from plasmids which contain LEU2 and URA3 gene (3). For this purpose, a leu2ura3 mutant strain of S. cerevisiae is transformed with LEU2 and URA3 containing plasmid and the transformants can be selected by growing the cells on SD Growth Medium w/o LEU-URA.