SD Growth Medium w/o URA includes a yeast nitrogen base along with ammonium sulfate, and dextrose as the carbon source, which is further supplemented with various amino acids except uracil which makes it a dropout growth medium for yeast cells allowing only uracil prototrophic yeast strain to grow. It is used for selection of auxotrophic phenotypes in yeast, selection for plasmid after yeast transformation, for yeast one/two hybrid screening.