SD Growth Medium w/o HIS includes a yeast nitrogen base along with ammonium sulfate, and dextrose as the carbon source, which is further supplemented with various amino acids except histidine which makes it a dropout growth medium for yeast cells allowing only histidine prototrophic yeast strain to gorw. It is used for selection of auxotrophic phenotypes in yeast, selection for plasmid after yeast transformation, for yeast one/two hybrid screening.