Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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SDA Growth Medium is a synthetic defined agar media for the growth of Saccharomyces cerevisiae.
Composition**
| Ingredients | Grams/Litre |
|---|---|
| Potassium dihyrogen phosphate | 1.00 |
| Magnesium sulphate | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride | 0.10 |
| Biotin | 0.002 mg |
| Calcium pantothenate | 0.4 mg |
| Folic acid | 0.002 mg |
| Inositol | 2.00 mg |
| Niacin | 0.4 mg |
| PABA | 0.2 mg |
| Pyridoxin, HCI | 0.4 mg |
| Riboflavin | 0.2 mg |
| Thiamine HCl | 0.4 mg |
| Boric acid | 0.5 mg |
| Copper sulphate | 0.04 mg |
| Potassium iodide | 0.1 mg |
| Ferric chloride | 0.2 mg |
| Manganese sulphate | 0.4 mg |
| Sodium molybdate | 0.2 mg |
| Zinc sulphate | 0.4 mg |
| Ammonium sulphate | 5.00 |
| Dextrose | 20.00 |
| Adenine | 0.010 |
| L-Arginine HCl | 0.050 |
| L-Aspartic acid | 0.080 |
| L-Histidine HCl | 0.020 |
| L-Isoleucine | 0.050 |
| L-Leucine | 0.100 |
| L-Lysine HCl | 0.050 |
| L-Methionine | 0.020 |
| L-Phenylalanine | 0.050 |
| L-Threonine | 0.100 |
| L-Tryptophan | 0.050 |
| L-Tyrosine | 0.050 |
| Uracil | 0.020 |
| L-Valine | 0.140 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 42.49 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
SDA Growth Medium is a synthetic defined agar media for the growth of Saccharomyces cerevisiae. Synthetically Defined media known as Yeast Nitrogen Base Media for the growth of yeast cells were first cited by Wickerham (1, 2). SDA Growth Medium includes a yeast nitrogen base along with ammonium sulfate and dextrose as the carbon source, which is further supplemented with various nutrients. This makes it a complete synthetic growth medium for yeast cells.
Quality Control
Appearance of Powder : Off white to yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity : Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC 9763 | good-luxuriant |
Storage and Shelf-life
Upon receipt, store at 2 - 8°C. Use before expiry date on the label.
| Product Name | SDA Growth Medium |
|---|---|
| SKU | G063 |
| Customized Product Available | No |
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