Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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MMA Growth Agar is a minimal defined media for the growth of Schizosaccharomyces pombe.
Principle and Interpretation
MMA Growth Agar is a minimal defined media for the growth of Schizosaccharomyces pombe. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. The fission yeast Schizosaccharomyces pombe is a model eukaryote which is very useful in studies of cell cycle and chromosome dynamics. These cells maintain their shape by growing through the cell tips and divide by medial fission to produce two daughter cells of equal sizes that makes them a powerful tool in cell cycle research. It was first developed as an experimental model in the 1950's for studying genetics (1, 2) and for studying the cell cycle (3, 4). MMA Growth Agar is used for the maintenance and propagation of S. pombe in various molecular microbiology procedures. MMA Growth Agar functions as a complete defined medium for fission yeast growth and contains salts, vitamins, trace elements and other supplements. It contains glucose as the carbon source.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Glucose | 10.00 |
| Potassium dihydrogen phosphate | 1.00 |
| Magnesium sulphate, 7H2O | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride, 2H2O | 0.10 |
| Ammonium sulphate | 5.00 |
| Boric acid | 500 mcg |
| Copper sulphate, 5H2O | 40 mcg |
| Potassium iodide | 100 mcg |
| Ferric chloride, 6H2O | 200 mcg |
| Manganese sulphate, H2O | 400 mcg |
| Sodium molybdate, 2H2O | 200 mcg |
| Zinc sulphate, 7H2O | 400 mcg |
| Biotin | 10 mcg |
| Calcium pantothenate | 0.001 |
| Nicotinic acid | 0.010 |
| Myoinositol | 0.010 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.72 grams in 1000 ml distilled water. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation :
MMA Growth Agar is a minimal defined media for the growth of Schizosaccharomyces pombe. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. The fission yeast Schizosaccharomyces pombe is a model eukaryote which is very useful in studies of cell cycle and chromosome dynamics. These cells maintain their shape by growing through the cell tips and divide by medial fission to produce two daughter cells of equal sizes that makes them a powerful tool in cell cycle research. It was first developed as an experimental model in the 1950's for studying genetics (1, 2) and for studying the cell cycle (3, 4). MMA Growth Agar is used for the maintenance and propagation of S. pombe in various molecular microbiology procedures. MMA Growth Agar functions as a complete defined medium for fission yeast growth and contains salts, vitamins, trace elements and other supplements. It contains glucose as the carbon source.
Quality Control
Appearance of Powder :
Light yellow coloured, homogeneous, free flowing powder.
Gelling:
Firm, comparable with 1.5% Agar gel.
Colour and Clarity :
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response :
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Schizosaccharomyces pombe | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | MMA Growth Agar |
|---|---|
| SKU | G060 |
| Customized Product Available | No |
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