Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
MB Growth Medium is a minimal defined media for the growth of Schizosaccharomyces pombe.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Glucose | 5.00 |
| Potassium dihydrogen phosphate | 0.50 |
| Potassium acetate | 0.36 |
| Magnesium sulphate, 7H2O | 0.50 |
| Sodium chloride | 0.10 |
| Calcium chloride, 2H2O | 0.10 |
| Ammonium sulphate | 5.00 |
| Boric acid | 500 mcg |
| Copper sulphate, 5H2O | 40 mcg |
| Potassium iodide | 100 mcg |
| Ferric chloride, 6H2O | 200 mcg |
| Manganese sulphate, H2O | 400 mcg |
| Sodium molybdate, 2H2O | 200 mcg |
| Zinc sulphate, 7H2O | 400 mcg |
| Biotin | 10 mcg |
| Calcium pantothenate | 0.001 |
| Nicotinic acid | 0.010 |
| Myoinositol | 0.010 |
| Uracil | 0.150 |
| Leucine | 0.150 (for leucine strains) |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 12 grams in 1000 ml distilled water. Sterilize by filtration through a 0.22µm filter or autoclave at 10 lbs pressure (115°C) for 20 minutes. Mix well and dispense as desired.
Principle and Interpretation
MB Growth Medium is a minimal defined media for the growth of Schizosaccharomyces pombe. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. The fission yeast Schizosaccharomyces pombe is a model eukaryote which is very useful in studies of cell cycle and chromosome dynamics. These cells maintain their shape by growing through the cell tips and divide by medial fission to produce two daughter cells of equal sizes that makes them a powerful tool in cell cycle research. It was first developed as an experimental model in the 1950's for studying genetics (1, 2) and for studying the cell cycle (3, 4). MB Growth Medium is used for the maintenance and propagation of S. pombe in various molecular microbiology procedures. It functions as a defined medium for fission yeast growth and it contains glucose and other supplements. Glucose serves as the carbon source.
Quality Control
Appearance of Powder : Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Light yellow coloured, clear solution without any precipitate.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Schizosaccharomyces pombe | good-luxuriant |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | MB Growth Medium |
|---|---|
| SKU | G059 |
| Customized Product Available | No |
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