Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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EMM Growth Agar with Dextrose is a minimal defined media for the growth of Schizosaccharomyces pombe
Composition**
| Ingredients | Grams/Litre |
|---|---|
| Phthalic acid K+ | 3.00 |
| Disodium hydrogen phosphate | 2.20 |
| Ammonium chloride | 5.00 |
| Dextrose | 5.00 |
| Magnesium chloride, 6H2O | 1.05 |
| Calcium chloride, 2H2O | 0.0147 |
| Potassium chloride | 1.00 |
| Sodium sulphate | 0.04 |
| Pantothenic acid | 0.001 |
| Nicotinic acid | 0.01 |
| Myoinositol | 0.01 |
| Biotin | 0.001 |
| Boric acid | 0.0005 |
| Manganese sulphate | 0.0004 |
| Zinc sulphate, 7H2O | 0.0004 |
| Ferric chloride, 6H2O | 0.0002 |
| Molybdic acid | 40 mcg |
| Potassium iodide | 0.0001 |
| Copper sulphate, 5H2O | 40 mcg |
| Citric acid | 0.001 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 32.32 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle and Interpretation
EMM Growth Agar with Dextrose is a minimal defined media for the growth of Schizosaccharomyces pombe.
Quality Control
Appearance of Powder :
Light yellow coloured, homogeneous, free flowing powder.
Gelling :
Firm, comparable with 1.5% Agar gel.
Colour and Clarity :
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response :
Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Schizosaccharomyces pombe | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | EMM Growth Agar with Low Dextrose |
|---|---|
| SKU | G058 |
| Customized Product Available | No |
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