Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition
| Ingredients | Grams/Litre |
|---|---|
| Phthalic acid K+ | 3.00 |
| Disodium hydrogen phosphate | 2.20 |
| Ammonium chloride | 5.00 |
| Dextrose | 20.00 |
| Magnesium chloride, 6H2O | 1.05 |
| Calcium chloride, 2H2O | 0.0147 |
| Potassium chloride | 1.00 |
| Sodium sulphate | 0.04 |
| Pantothenic acid | 0.001 |
| Nicotinic acid | 0.01 |
| Myoinositol | 0.01 |
| Biotin | 0.001 |
| Boric acid | 0.0005 |
| Manganese sulphate | 0.0004 |
| Zinc sulphate, 7H2O | 0.0004 |
| Ferric chloride, 6H2O | 0.0002 |
| Molybdic acid | 40 mcg |
| Potassium iodide | 0.0001 |
| Copper sulphate, 5H2O | 40 mcg |
| Citric acid | 0.001 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.32 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Cool to 45-50°C. Mix well and dispense as desired.
Principle and Interpretation
EMM Growth Agar is a minimal defined media for the growth of Schizosaccharomyces pombe. Yeasts are unicellular eukaryotes and extensively studied model organism in molecular genetics. The fission yeast Schizosaccharomyces pombe is a model eukaryote which is very useful in studies of cell cycle and chromosome dynamics. These cells maintain their shape by growing through the cell tips and divide by medial fission to produce two daughter cells of equal sizes that makes them a powerful tool in cell cycle research. It was first developed as an experimental model in the 1950's for studying genetics (1, 2) and for studying the cell cycle (3, 4). EMM (Edinburgh Minimal Media) Growth Medium is used for the maintenance and propagation of S. pombe in various molecular microbiology procedures. It functions as a minimal defined medium for fission yeast growth and it contains dextrose, minerals and trace elements. Dextrose serves as the carbon source.
Quality Control
Appearance of Powder : Light yellow coloured, homogeneous, free flowing powder.
Gelling : Firm, comparable with 1.5% Agar gel.
Colour and Clarity : Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response : Cultural characteristics observed after an incubation at 25-30°C for 18 - 48 hours.
Cultural Response
| Organisms (ATCC) | Growth |
|---|---|
| Schizosaccharomyces pombe | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
Do not use if package is damaged.
| Product Name | EMM Growth Agar |
|---|---|
| SKU | G052 |
| Customized Product Available | No |
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