Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
YES Growth Medium is used for the vegetative growth of Schizosaccharomyces pombe.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Yeast extract | 5.00 |
| Dextrose | 30.00 |
| Adenine | 0.05 |
| Histidine | 0.05 |
| Leucine | 0.05 |
| Lysine | 0.05 |
| Uracil | 0.05 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 50.25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and pour into sterile Petri plates.
Principle and Interpretation
YES Growth Agar is used for the vegetative growth of Schizosaccharomyces pombe.
Schizosaccharomyces pombe, also called "fission yeast", is a species of yeast. It is used as a model organism in molecular and cell biology. It is possibly the eukaryote with the shortest genome. These cells maintain their shape by growing through the cell tips and divide by medial fission to produce two daughter cells of equal sizes that makes them a powerful tool in cell cycle research. It was first developed as an experimental model in the 1950's for studying genetics (1, 2) and for studying the cell cycle (3, 4).
YES functions as a complete medium for fission yeast growth and it contains yeast extract, glucose and other supplements which include adenine, histidine, leucine, uracil and lysine hydrochloride. Dextrose serves as the carbon source. The generation time of a wild-type strain of fission yeast is about 2 hours in YES (5). Since the medium contains 30 grams per litre dextrose, the medium should be properly dissolved in water before autoclaving to avoid caramelization of the sugar.
Quality Control
Appearance of Powder : Light yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity : Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response : Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Schizosaccharomyces pombe | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | YES Growth Agar |
|---|---|
| SKU | G048 |
| Customized Product Available | No |
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