Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
Presporulation Growth Medium is used for the growth and sporulation of Saccharomyces cerevisiae.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Peptone | 3.00 |
| Yeast extract | 8.00 |
| Potassium acetate | 20.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Presporulation Growth Medium is used for the growth and sporulation of Saccharomyces cerevisiae. Pre-Sporulation Medium is used for strains that do not sporulate well when grown on sporulation medium directly. Saccharomyces cerevisiae is a unicellular eukaryote that has become an important tool in microbial genetic techniques. It undergoes meiosis and sporulation which takes place in a single cell. Presporulation medium stimulates the sporulation of diploid yeast cells which occurs only when cells are deprived of any carbon source. Potassium acetate enhances the sporulation of diploid strains. Cells are first exposed to the pre-sporulation medium for 1-2 days before transferring them to sporulation medium.
Quality Control
Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity: Light yellow coloured, clear solution without any precipitate.
Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Saccharomyces cerevisiae ATCC9763 | good-luxuriant |
Storage and Shelf Life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Presporulation Growth Medium |
|---|---|
| SKU | G041 |
| Customized Product Available | No |
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