Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
2 XYT Growth Medium is an optimized formulation for the cultivation of recombinant Escherichia coli.
Composition**
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 16.00 |
| Yeast extract | 10.00 |
| Sodium chloride | 5.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
2 XYT Growth Medium is an optimized formulation for the the cultivation of recombinant Escherichia coli. This media is 2 times richer than the YT media. This media was originally formulated as a nutritionally enriched growth medium for growth of recombinant strains of Escherichia coli and can also be used for propagation of M13 bacteriophage (1-3). It permits larger quantity of phage production without exhausting the host. Yeast extract and tryptone provide all the required amino acids, nucleotide precursors, vitamins and other metabolites and as a result the cells grow faster in this medium. Sodium chloride provides sodium ions for transport and osmotic balance.
Quality Control
Appearance of Powder : Cream to yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Light yellow coloured, clear solution without any precipitate.
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli ATCC 23724 | good-luxuriant |
| Escherichia coli ATCC 25922 | good-luxuriant |
| Escherichia coli MTCC 1652 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | 2XYT Growth Medium |
|---|---|
| SKU | G034 |
| Customized Product Available | No |
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