Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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YT Growth Top Agar is an optimized formulation for the cultivation of recombinant Escherichia coli.
Composition** :
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 8.00 |
| Yeast extract | 5.00 |
| Sodium chloride | 5.00 |
| Agar | 7.00 |
** Formula adjusted, standardized to suit performance parameters
Directions:
Suspend 25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle and Interpretation :
YT Growth Top Agar is an optimized formulation for the cultivation of recombinant Escherichia coli. This media was originally formulated as a nutritionally enriched growth medium for growth of recombinant strains of Escherichia coli and can also be used for propagation of M13 bacteriophage (1-3). It permits larger quantity of phage production without exhausting the host. Yeast extract and tryptone provide all the required amino acids, nucleotide precursors, vitamins and other metabolites and as a result the cells grow faster in this medium. Sodium chloride provides sodium ions for transport and osmotic balance. This medium contains 0.7% of agar which functions as the top agar for the propagation of bacteriophages.
Quality Control :
Appearance of Powder :
Cream to yellow coloured, homogeneous, free flowing powder.
Gelling:
Firm, comparable with 0.7% Agar gel.
Colour and Clarity :
Clear to slightly opalescent gel forms in Petri plates.
Cultural Response :
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli ATCC 23724 | good-luxuriant |
| Escherichia coli ATCC 25922 | good-luxuriant |
| Escherichia coli MTCC1652 | good-luxuriant |
Storage and Shelf-life :
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | YT Growth Top Agar |
|---|---|
| SKU | G033 |
| Customized Product Available | No |
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