NZYDT Growth Medium

Principle and Interpretation

NZYDT Growth Medium is used for cultivation of recombinant Escherichia coli strains. This medium was developed by Blattner and colleagues as a rich medium for the propagation of bacteriophages which infects its cell host, E. coli for replication (1). Cells grow very fast in this medium as this medium provides all the amino acids, vitamins and other metabolites required for cell growth (2). Casein enzymic hydrolysate provides nitrogen, amino acids, and carbon sources for the cells. Yeast extract functions as the source of vitamins and trace elements. Sodium chloride provides sodium ions for transport and osmotic balance and Magnesium sulfate is a source of magnesium ions required in a variety of enzymatic reactions, including DNA replication (3). NZYDT Growth Medium allows the cells to grow more rapidly as they do not have to synthesize nucleotide precursors and other factors required for growth.