Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Tryptone Growth Top Agar
Tryptone Grrowth top agar II is used as a general purpose media for the growth and maintenance of Escherichia coli where Tryptone provides all the required peptides and
peptones.
Your enquiry has been submitted
Please login to Post an Enquiry.
Intended Use
Tryptone Growth Top Agar is used as a general purpose media for the growth of Escherichia coli.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 10.00 |
| Sodium chloride | 8.00 |
| Agar | 7.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle and Interpretation
Tryptone Growth Top Agar is used as a general purpose media for the growth of Escherichia coli. Tryptone provides all the required peptides and peptones. Sodium chloride provides the sodium ions which help in the membrane transport and maintenance of osmotic equilibrium of the medium. Top agar is used to distribute bacteriophage and bacterial cells uniformly on the thin layer over the surface of a plate. Top agar contains less amount of agar than usual plates and so stays in a molten state for several days when it is kept at 45° to 50°C.
Quality Control
Appearance of Powder: Light yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 0.7% Agar gel.
Colour and Clarity: Light yellow coloured, clear to slight opalescent gel forms in tubes.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Growth Promoting Properties
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli ATCC 23724 | good-luxuriant |
| Escherichia coli ATCC 25922 | good-luxuriant |
| Escherichia coli MTCC1652 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Tryptone Growth Top Agar |
|---|---|
| SKU | G012 |
| Customized Product Available | No |
Write Your Own Review