Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Tryptone Growth Agar II
Tryptone Grrowth agar II is used as a general purpose media for the growth and maintenance of Escherichia coli where Tryptone provides all the required peptides and
peptones.
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Intended Use
Tryptone Growth Agar II is used as a general purpose media for the growth of Escherichia coli.
Composition** :
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 10.00 |
| Sodium chloride | 8.00 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions:
Suspend 33 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well and dispense as desired.
Principle and Interpretation :
Tryptone Growth Agar II is used as a general purpose media for the growth of Escherichia coli. Tryptone provides all the required peptides and peptones. Sodium chloride provides the sodium ions which help in the membrane transport and maintenance of osmotic equilibrium of the medium.
Quality Control :
Appearance of Powder : Cream to yellow coloured, homogeneous, free flowing powder.
Gelling : Firm, comparable with 1.5% Agar gel.
Colour and Clarity : Light yellow coloured, clear to slight opalescent gel forms in petriplates.
Cultural Response : Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50-100 | good-luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | good-luxuriant |
| Escherichia coli MTCC 1652 | 50 - 100 | good-luxuriant |
Storage and Shelf-life :
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Tryptone Growth Agar II |
|---|---|
| SKU | G011 |
| Customized Product Available | No |
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