Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Terrific Growth Medium is recommended for high density of Escherichia coli host cells and higher yield of plasmid DNA.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 12.00 |
| Yeast extract | 24.00 |
| Potassium dihydrogen phosphate | 2.2 |
| Dipotassium phosphate | 9.4 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 47.6 grams in 1000 ml distilled water containing 0.4 ml v/v glycerol. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
Terrific Growth Medium is recommended for high density of Escherichia coli host cells and higher yield of plasmid DNA. It was first formulated by Tartoff and Hobbs as an enriched medium for E. coli compared to LB and Tryptone Media (1). The medium was developed to enhance growth of E. coli recombinant strains and to obtain higher yield of plasmid DNA compared to LB and Tryptone broth (2). Due to the presence of large amount of tryptone and yeast extract in this medium plasmid yield is very high. Potassium dihydrogen phosphate prevents any drop in pH of the medium during bacterial growth. Moreover, tryptone and yeast extract provide additional nutrients and growth factors required for improved growth of E. coli recombinant strains. Glycerol is added in the medium as the carbon source and it is not fermented to acetic acid like glucose.
Quality Control
Appearance of Powder : Light yellow coloured, homogeneous, free flowing powder.
Colour and Clarity : Light amber coloured, clear solution without any precipitate.
Cultural Response : Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
References
- Tartoff, K. D. and C. A. Hobbs. 1987. Improved media for growing plasmid and cosmid clones. Bethesda Research Laboratories Focus. 9:12.
- Sambrook, Fritsch and Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
| Product Name | Terrific Growth Medium |
|---|---|
| SKU | G004 |
| Customized Product Available | No |
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