Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Composition**
| Ingredients | Gms / Litre |
|---|---|
| Yeast extract | 3.000 |
| L-Lysine | 5.000 |
| Carbohydrate source | 18.500 |
| Sodium chloride | 5.000 |
| Bile salts | 2.500 |
| Sodium thiosulphate | 6.800 |
| Ferric ammonium citrate | 0.800 |
| Phenol red | 0.080 |
| Agar | 15.000 |
Final pH (at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch in the protected area. Remove the wrappping and open the lid and carefully lift up the enclosed prepared medium plate so as to avoid touching the agar surface by hand. Touch the surface of agar plate onto the surface to be tested. Gently press the plate manually for upto 10 sencond. Apply constant and uniform pressure to the whole surface (ensuring that an even pressure of 25 gm/cm2 is distributed over the whole plate for 10 seconds). Replace exposed medium plate back in the base plate. Close the lid. Press the sides of the lid to make sure that it is fixed in the grooves. Disinfect the surface where the sample was taken in order to remove any possible traces of agar. Incubate the plates at specified temperature. After incubation as recommended count the number of colonies which have appeared on the surface of medium. Alternative Methods of Inoculation:
- To use as Culture Plate (ii)
- Sample Dilution Plate (iii)
- Swabbing Plate (iv)
- To use as Gravitation Settling Plate (v)
Principle And Interpretation
HiTouch Flexi plates are ready to use sterile media supplied in flexible disposable plates, 55 mm in diameter. It is grid scored on the base and is irradiated to ensure perfect sterility. Medium is filled aseptically and each plate is packed in pre-sterilized plastic bag. HiTouch Flexi plate is then packed in plastic pouch wrapping. The unique flexible plate configuration ensures close contact even with uneven surfaces. where not only counts are obtained but it is also possible to select and differentiate between groups of microorganisms like coliforms (both E. coli and non E. coli). These plates are specially developed for microbial testing, The Flexi plate medium formula is suitable for selective isolation and enumeration of S. typhi and other species and the grids enable direct reading on the plates of the number of colonies per cm2.
Quality Control
Appearance: Sterile plastic plate containing red coloured firm gel.
Quantity of medium: 9ml of gel in plastic plate.
Reaction: 7.20-7.60
Sterility Test: Passes release criteria
Cultural response
Cultural characteristics after incubation at 35-37°C for 18-24 hours.
| Organism | Growth | Colour of Colony |
|---|---|---|
| Enterobacter aerogenes ATCC 13048 | Fair | Yellow |
| Proteus mirabilis ATCC 25933 | Good-luxuriant | grey with black centres |
| Proteus vulgaris ATCC 13315 | Good-luxuriant | grey with black centres |
| Escherichia coli ATCC 25922 | Fair | Yellow |
| Staphylococcus aureus ATCC 25923 | Inhibited | - |
| Shigella dysenteriae ATCC 13313 | Good-luxuriant | Red |
| S. serotype enteritidis ATCC 13076 | Good-luxuriant | Red with black centres |
| S. serotype paratyphi A ATCC 9150 | Good-luxuriant | Red |
| S. serotype paratyphi B ATCC 8759 | Good-luxuriant | Red with black centres |
| S. serotype typhi ATCC 6539 | Good-luxuriant | Red with black centres |
| S. serotype typhimurium ATCC 14028 | Good-luxuriant | Red with black centres |
| Shigella sonnei ATCC 25931 | Good-luxuriant | Red |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | HiTouch™ Salmonella Count Flexi Plate™ |
|---|---|
| SKU | FL023 |
| Customized Product Available | No |
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