Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Intended Use
For enumeration (count) of coliform bacteria.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Biopeptone | 5.000 |
| Yeast extract | 3.000 |
| Lactose | 10.000 |
| D-Mannitol | 5.000 |
| Sodium chloride | 7.500 |
| Sodium lauryl sulphate | 0.200 |
| Sodium deoxycholate | 0.100 |
| Indicator dye | 0.650 |
| Agar | 15.000 |
| Final pH (at 25°C) | 7.2±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Open the pouch in the protected area. Remove the wrappping and open the lid and carefully lift up the enclosed prepared medium plate so as to avoid touching the agar surface by hand. Touch the surface of agar plate onto the surface to be tested. Gently press the plate manually for upto 10 sencond. Apply constant and uniform pressure to the whole surface (ensuring that an even pressure of 25 gm/cm2 is distributed over the whole plate for 10 seconds). Replace exposed medium plate back in the base plate. Close the lid. Press the sides of the lid to make sure that it is fixed in the grooves. Disinfect the surface where the sample was taken in order to remove any possible traces of agar. Incubate the plates at specified temperature. After incubation as recommended count the number of colonies which have appeared on the surface of medium.
Alternative Methods of Inoculation:
- To use as Culture Plate (ii)
- Sample Dilution Plate (iii)
- Swabbing Plate (iv)
- To use as Gravitation Settling Plate (v)
Principle And Interpretation
HiTouch Flexi plates are ready to use sterile media supplied in flexible disposable plates, 55 mm in diameter. It is grid scored on the base and is irradiated to ensure perfect sterility. Medium is filled aseptically and each plate is packed in pre-sterilized plastic bag. HiTouch Flexi plate is then packed in plastic pouch wrapping. The unique flexible plate configuration ensures close contact even with uneven surfaces. where not only counts are obtained but it is also possible to select and differentiate between groups of microorganisms like coliforms (both E. coli and non E. coli). These plates are specially developed for microbial testing, The Flexi plate medium formula is suitable for enumeration of coliform bacteria and the grids enable direct reading on the plates of the number of colonies per cm2.
Quality Control
Appearance: Sterile plastic plate containing deep pinkish purple coloured firm gel
Quantity of Medium: 9ml of gel in plastic plate
Reaction: 7.00-7.40
Sterility test: Passes release criteria
Cultural Response
Cultural characteristics was observed after incubation at 41.5°C for 7-18 hours.
| Organism | Growth | Colour of Colony |
|---|---|---|
| Escherichia coli ATCC 25922 | Luxuriant | Yellow with yellow zones |
| Staphylococcus aureus ATCC 25923 | Inhibited | - |
| Enterococcus faecalis ATCC 29212 | Inhibited | - |
Storage and Shelf Life
Store between 2-8°C. Use before expiry date on the label.
| Product Name | HiTouch™ Rapid Coliform Count Flexi Plate™ |
|---|---|
| SKU | FL005 |
| Customized Product Available | No |
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