Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Ready Prepared Collection and Transport Media#CC293D
Intended Use:
Recommended for transporting microbial specimens in presence of antiseptics and disinfectants.
Composition
| Ingredients | g/L |
|---|---|
| Tryptone | 5.000 |
| Yeast extract | 2.500 |
| Dextrose (Glucose) | 10.000 |
| Sodium thioglycollate | 1.000 |
| Sodium thiosulphate | 6.000 |
| Sodium bisulphite | 2.500 |
| Lecithin | 7.000 |
| Polysorbate 80 (Tween 80) | 5.000 |
| Final pH (at 25°C) | 7.6±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Using the capped swab provided along with the Saline containing tube, collect the sample or specimen from surface. Discard the cap of the tube and insert the capped swab with the sample till the bottom of the medium. Tighten the cap firmly. The specimen will be preserved during transportation and also the viability of the organisms will be maintained. Some growth of contaminants may occur during longer period of transport. After the transportation, the specimen should be inoculated in proper medium as soon as possible.
Principle And Interpretation
Dey-Engley Neutralizing media is formulated as per the procedure described by Engley and Dey (1). The medium neutralizes a broad spectrum of antiseptics and disinfectants including quaternary ammonium compounds, phenolics, iodine and chlorine preparations, mercurials, formaldehyde and glutaraldehyde. Sodium thioglycollate, sodium thiosulphate, sodium bisulphate, soya lecithin and polysorbate 80 act as neutralizing components. Sterile viscous swabs allow absorption of specimen material while polystyrene shaft allows semiflexibility to the swab stick, aiding in collection.
Type of specimen
Environmental samples
Specimen Collection and Handling
For environmental samples follow appropriate techniques for handling specimens as per established guidelines (2,3). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Further biochemical and serological tests must be carried out for complete identification.
- Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user's unique requirement.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance: Sterile Dey-Engley Neutralizing Broth (w/o Bromo cresol purple) in tubes with sterile cotton swabs.
Colour of the medium: Yellow coloured medium.
Quantity of the medium: 8 ml of medium in polystyrene tubes
pH: 7.40 -7.80
Sterility check: Passes release criteria
Cultural Response
Viability of following organisms was established for a period of 48 hours. Organisms grew luxuriantly when recovered on Tryptone Soya Agar (M290) and incubated at 35 - 37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| ** Bacillus spizizenii ATCC 6633 (00003*) | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 (00013*) | 50-100 | luxuriant |
| Pseudomonas aeruginosa ATCC 27853 (00025*) | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 (00031*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp.aureus ATCC 25923 (00034*) | 50-100 | luxuriant |
| Escherichia coli ATCC 8739 (00012*) | 50-100 | luxuriant |
| Staphylococcus aureus subsp.aureus ATCC 6538 (00032*) | 50-100 | luxuriant |
Key: (*) Corresponding WDCM numbers, (**) Formerly known as Bacillus subtilis subsp. spizizenii
Storage and Shelf life :
Store between 5-30°C with caps firmly screwed. Use before expiry date on the label. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).
| Product Name | HiCulture™ Transport Swabs w/ Dey-Engley Neutralizing Broth |
|---|---|
| SKU | MS1062 |
| Customized Product Available | No |
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