Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Sterility Testing#CC293D
Composition
| Ingredients | Gms/Litre |
|---|---|
| Peptone | 10.000 |
| Beef extract | 10.000 |
| Sodium chloride | 5.000 |
| pH after sterilization | 7.3±0.1 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 25 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Sterilise by autoclaving at 10 lbs pressure (115°C) for 30 minutes or alternatively at 15lbs pressure(121°C) for 15 minutes or as per validated cycle
Principle And Interpretation
Nutrient Broth Medium is a general purpose medium used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Wastewater (1) and Dairy Products (2) in accordance with IP (4). It can also be used for cultivating several less fastidious microorganisms.
Beef extract and peptone provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients to the non-fastidious organisms. Sodium chloride maintains osmotic equilibrium of the medium.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light yellow coloured clear solution.
pH
7.20-7.40
Growth Promotion Test
Growth promotion is carried out as per Indian Pharmacopoeia
Cultural Response
MM244: Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 8739 | 50-100 | luxuriant |
| Escherichia coli NCTC 9002 | 50-100 | luxuriant |
| Salmonella Typhimurium ATCC 14028 | 50-100 | luxuriant |
| Salmonella Abony NCTC 6017 | 50-100 | luxuriant |
| Peudomonas aeruginosa ATCC 9027 | 50-100 | luxuriant |
| Escherichia coli ATCC 25922 | 50-100 | luxuriant |
| Peudomonas aeruginosa ATCC 27853 | 50-100 | luxuriant |
| Enterobacter aerogenes ATCC 13048 | 50-100 | luxuriant |
| Klebsiella pneumoniae ATCC 13883 | 50-100 | luxuriant |
Storage and Shelf Life
Store below 30°C in tightly closed container and use freshly prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Nutrient Broth |
|---|---|
| SKU | MM244 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1.Greenberg A.E., Trussell R.R. and Clesceri L.S. (Eds.), 1985, Standard Methods for the Examination of Water andWastewater, 16th ed., APHA, Washington, D.C. 2.American Public Health Association, 1978, Standard Methods for the Examination of Dairy Products,14th ed., APHA, Inc.,Washington, D.C. 3.American Public Health Association, 1917, Standard Methods of Water Analysis, 3rd ed., APHA, Inc., Washington, D.C. 4.Indian Pharmacopoeia, 1985, Third Edition, Government of India Ministry of Health of family Welfare |
| Customized Product Available | No |
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