Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Growth Media for Escherichia coli#CC293D
Intended Use
LB Growth Agar for Lambda is used for lambda phage.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 10.00 |
| Yeast extract | 5.00 |
| Sodium chloride | 10.00 |
| MgSO4. 7H2O | 2.00 |
| Agar | 15.00 |
Formula adjusted, standardized to suit performance parameters
Directions
Suspend 40.97 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
LB Growth Agar for Lambda is used for lambda phage. The three main components of this media are tryptone, yeast extract and sodium chloride. Tryptone and yeast extract provide nitrogenous compounds, vitamin B complex and other essential growth nutrients. Sodium chloride maintains osmotic equilibrium. In LB Growth Agar media Mg2+ is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2+ for optimal adsorption.
Quality Control
Appearance of Powder: Cream to yellow colored, homogeneous, free flowing powder.
Color and Clarity: Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Cultural Response: Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Organisms (ATCC)
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli ATCC 23724 | good-luxuriant |
| Escherichia coli ATCC 25922 | good-luxuriant |
| Escherichia coli MTCC1652 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | LB Growth Agar For Lambda |
|---|---|
| SKU | G030 |
| Customized Product Available | No |
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