Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
Growth Media for Escherichia coli#CC293D
SOB Growth Agar is used for preparing competent cells prior to transformation.
Composition
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 20.00 |
| Yeast extract | 5.00 |
| Sodium chloride | 0.50 |
| Magnesium sulphate, heptahydrate | 5.00 |
| Agar | 15.00 |
** Formula adjusted, standardized to suit performance parameters
Directions
Suspend 42.94 grams of dehydrated medium in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation
SOB Growth Agar is used for preparing competent cells prior to transformation. Super Optimal Broth (SOB medium) Growth Medium was first formulated by Douglas Hanahan in 1983 as a nutritionally enriched growth media for bacteria, especially Escherichia coli (1). It is used in the preparation and transformation of chemically competent cells. E. coli cells are first made competent during transformation where perforations are made in the bacterial cells so that the foreign DNA can penetrate the cells (2). To endure this process, competent cells require a rich isotonic medium. Tryptone provides nitrogen, amino acids and other growth factors which permit the cells to go through the stress of transformation. Vitamins and trace elements are contained in Yeast Extract. Sodium chloride provides essential ions for transport and osmotic balance. Magnesium sulfate provides magnesium ions which are required in a variety of enzymatic reactions, including DNA replication (2). This media contains agar as the solidifying agent.
Quality Control
Appearance of Powder: Cream to yellow coloured, homogeneous, free flowing powder.
Gelling: Firm, comparable with 1.5% Agar gel.
Colour and Clarity: Light amber coloured, clear to slightly opalescent gel forms in petri plates.
Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Growth |
|---|---|
| Escherichia coli ATCC 23724 | good-luxuriant |
| Escherichia coli ATCC 25922 | good-luxuriant |
| Escherichia coli MTCC1652 | good-luxuriant |
Storage and Shelf-life
Store below 30°C and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | SOB Growth Agar |
|---|---|
| SKU | G016 |
| Customized Product Available | No |
Write Your Own Review