Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
Your enquiry has been submitted
Something problem with the server. Try again.
LB Growth Medium w/o Sodium Chloride
LB-Growth Medium w/o Sodium chloride is used in protein expression systems where Sodium chloride is used to induce protein.
Your enquiry has been submitted
Please login to Post an Enquiry.
Growth Media for Escherichia coli#CC293D
LB-Growth Medium w/o Sodium chloride is used in protein expression systems where Sodium chloride is used to induce protein.
Composition**:
| Ingredients | Grams/Litre |
|---|---|
| Tryptone | 10.00 |
| Yeast extract | 5.00 |
** Formula adjusted, standardized to suit performance parameters
Directions:
Suspend 15 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle and Interpretation:
LB-Growth Medium w/o Sodium chloride is used in protein expression systems where Sodium chloride is used to induce protein. LB Media, originally developed by Bertani, is a very common and nutritionally rich growth media for E. coli (1). This media is extensively used for the maintenance and propagation of plasmid DNA and for the growth of the recombinant strains which contain protein expression vector (2). The ingredients provide all the growth factors required for the E. coli strain. Tryptone provides all the required peptides and peptones. Yeast extract supplies all the essential vitamins, nucleotides, amino acids, carbohydrate and trace elements.
Quality Control:
Appearance of Powder:
Cream to yellow colored, homogeneous, free flowing powder.
Color and Clarity:
Light yellow colored, clear solution without any precipitate.
Cultural Response:
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
| Organisms (ATCC) | Inoculum (CFU) | Growth |
|---|---|---|
| Escherichia coli ATCC 23724 | 50 - 100 | good-luxuriant |
| Escherichia coli ATCC 25922 | 50 - 100 | good-luxuriant |
| Escherichia coli MTCC 1652 | 50 - 100 | good-luxuriant |
Storage and Shelf-life:
Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | LB Growth Medium w/o Sodium Chloride |
|---|---|
| SKU | G008 |
| Customized Product Available | No |
Write Your Own Review