Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Special Media for Fungi#CC293D
Intended Use
Recommended for cultivation of fungi.
Composition
| Ingredients | g/L |
|---|---|
| Corn Meal | 20.000 |
| Dextrose (Glucose) | 10.000 |
| Peptone | 10.000 |
| Yeast extract | 4.000 |
| Agar | 20.000 |
| Final pH (at 25°C) | 6.5±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 64.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.
Principle And Interpretation
Prospero and Reyes (1) investigated the use of Corn Meal Agar, Soil Extract Agar and Purified Polysaccharide Medium for the morphological identification of Candida albicans. Corn Meal Agar is a nutritionally rich medium so it may be also employed for the maintenance of stock cultures of fungi. Corn Meal Peptone Yeast Agar is prepared as per Benjamin 2) for the cultivation of fungi.The media contain corn meal, which enhances the growth of fungi. Peptone and yeast extract provide essential nutrients. Addition of dextrose to the medium supports more luxuriant growth of some fungi as compared to the medium without dextrose, but dextrose supplemented Corn Meal Agar should not be used for chlamydospores production.
Type of specimen
Clinical samples - skin scrapings, urine, etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (3,4).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic Use only. For professional use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- A non-selective and selective medium should be inoculated for isolation of fungi from conceivably contaminated specimens.
- Further biochemical and serological tests must be carried out for further identification.
Performance And Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous coarse powder
Gelling
Firm, comparable with 2.0% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, opalescent gel forms in Petri plates
Reaction
Reaction of 6.4% w/v aqueous solution at 25°C. pH : 6.5±0.2
Cultural Response
Cultural characteristics observed after an incubation at 23-27°C for upto 4 days (For observing Chlamydospore formation:Using a straight wire, make a deep cut in the Corn Meal Agar plate with inoculum. Place a flamed sterile coverslip over the line of inoculum. After incubation, the streaks are examined microscopically, through the coverslip, using low and high power objectives, for chlamydospore formation.)
| Organism | Inoculum (CFU) | Growth | Chlamydospores Recovery |
|---|---|---|---|
| #Aspergillus brasiliensis ATCC 16404 | 50-100 | luxuriant | negative |
| Candida albicans ATCC 10231 | 50-100 | luxuriant | positive >=70% |
| Saccharomyces cerevisiae ATCC 9763 | 50-100 | luxuriant | negative >=70% |
| Saccharomyces uvarum ATCC 28098 | 50-100 | luxuriant | negative >=70% |
Key:*Corresponding WDCM numbers.
# - Formerly known as Aspergillus niger
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (3,4).
| Product Name | Corn Meal Peptone Yeast Agar |
|---|---|
| SKU | M731 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Prospero, Magdalene T. and Reyes A. C., 1955, ActaMed, Phillipina 12(2), 69-742. 2.Benjamin R. K., 1958, Aliso, 4,150. 3.Booth C., (Ed.), 1971, Methods in Microbiology by J. R. Norris and D. W. Ribbons, Vol. 4, Academic Press, London 4.Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Edition 5.Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)Manual of Clinical Microbiology, 11th Edition. Vol. 1. |
| Customized Product Available | No |
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