Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Rhizobium and Other Root Nodulating Bacteria#CC293D
Intended Use
Recommended for detection and cultivation of nitrogen fixing bacteria.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Sucrose | 20.000 |
| Dipotassium hydrogen phosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Sodium chloride | 0.500 |
| Ferrous sulphate | 0.100 |
| Sodium molybdate | 0.005 |
| Calcium carbonate | 2.000 |
| Agar | 15.000 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 39.1 grams in 1000 ml purified / distilled water. Heat just to boiling. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50C. Mix well and pour into sterile Petri plates.
Note: Due to presence of calcium carbonate, the medium forms opalescent solution with white precipitate.
Principle And Interpretation
Nitrogen-fixing organisms are free-living bacteria, which grow well on a nitrogen-free medium. These bacteria utilize atmospheric nitrogen gas for their cell protein synthesis. This cell protein is then mineralized in soil after the death of the cells thereby contributing towards the nitrogen availability of the crop plants (5). Nitrogen fixing bacteria enter into symbiosis only with leguminous plants, by infecting their roots and forming nodules on them. Jensens Medium is formulated according to Jensen and is recommended for detection and cultivation of nitrogen fixing bacteria (2). Sucrose acts as the energy source. Sodium molybdate in the media increases the fixation of nitrogen (4).Sodium chloride maintains osmotic equilibrium of the media. Calcium stimulates nodulation when present as chloride or sulphate.
Type of specimen
Soil sample
Specimen Collection and Handling
For soil samples follow appropriate techniques for handling specimens as per established guidelines (5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
- Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
White to cream homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Cream coloured, slightly opalescent gel with a slight precipitate forms in Petri plates.
Cultural Response
Cultural characteristics observed after incubation at 30°C for 8 days.
| Organism | Growth |
|---|---|
| Rhizobium leguminosarum ATCC 10004 | luxuriant |
| Rhizobium meliloti ATCC 9930 | luxuriant |
| Rhizobium oryzae ATCC 9363 | luxuriant |
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,3).
| Product Name | Jensen’s Medium (Agar) |
|---|---|
| SKU | M710 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1.Subba Rao N. S., 1977, In: Soil Microorganisms and Plant Growth, Oxford and IBH Publishing Co., New Delhi, Pages254-255. 2.Jensen. H. L., 1942, Pro Line Soc. N.S.W., 57,205-212. 3.Ranganayaki S., Mohan C., Ally Z., 1981; 21 (8): 607-10. |
| Customized Product Available | No |
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