Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Staphylococcus aureus#CC293D
Intended Use
Recommended for studying fermentation by Staphylococcus species in accordance with FDA BAM, 1998.
Composition**
| Ingredients | Gms / Litre |
|---|---|
| Tryptone | 10.000 |
| Yeast extract | 1.000 |
| Dextrose (Glucose) | 10.000 |
| Bromocresol purple | 0.040 |
| Agar | 2.000 |
| Final pH (at 25°C) | 7.0±0.2 |
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 23.04 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving at 10 lbs pressure (115°C) for 20 minutes. Allow tubed medium to cool in an upright position.
Principle And Interpretation
Fermentation Medium for Staphylococcus and Micrococcus, w/ 0.2% Agar is used for studying the fermentation characteristics of Staphylococcus species in accordance with FDA BAM, 1998 (2). Staphylococci and Micrococci are the most frequently encountered cocci in the clinical laboratory. Both are gram positive and catalase positive. Ability to ferment glucose has served as the basis for differentiating staphylococci from the micrococci that lacks the ability to ferment glucose (1). Staphylococcus aureus is a primary pathogen, which may be associated with severe infection. Micrococci are generally strict aerobes and can reduce nitrate. Fermentation Medium for Staphylococcus and Micrococcus is recommended for differentiation of these two organisms on the basis of glucose fermentation (3,6).
Tryptone and yeast extract provide necessary nitrogenous nutrients for the organisms. Glucose is the fermentable carbohydrate source in the medium. Bromo cresol purple is the pH indicator. Incorporation of small amount of agar in this medium helps to create anaerobic condition in the depths of the tubes.
Type of specimen
Food samples
Specimen Collection and Handling
According to the BAM protocol, total plate count of the suspected sample is carried out using Baird Parker Agar (M043). Suspected colonies of S. aureus are inoculated into Fermentation Medium for Staphylococcus and Micrococcus, w/ 0.2% Agar. Make sure that the inoculum reaches the bottom of the tube. Overlay the surface of agar with a 25mm layer of sterile paraffin oil. Incubate the tubes for 5 days at 37°C. Acid production is indicated by the change in colour of the medium to yellow, indicating presence of S. aureus. Run controls simultaneously.
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
- Due to nutritional variation certain strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to greenish yellow homogeneous free flowing powder
Gelling
Semisolid, comparable with 0.2% Agar gel.
Colour and Clarity of prepared medium
Purple coloured clear to slightly opalescent gel forms in tubes as butts
Reaction
Reaction of 2.3% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-24 hours.
| Organism | Inoculum (CFU) | Growth | Acid production |
|---|---|---|---|
| Micrococcus luteus ATCC 10240 | 50-100 | good-luxuriant | negative reaction, no colour change |
| Staphylococcus aureus subsp. aureus ATCC 25923 (00034*) | 50-100 | good-luxuriant | positive reaction, yellow colour |
Key : * - Corresponding WDCM numbers
Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
| Product Name | Fermentation Medium For Staphylococcus and Micrococcus, w/ 0.2% Agar |
|---|---|
| SKU | M827F |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Animal |
| Packaging type | HDPE |
| References | 1. Smith K. J., Neafie R., Yeager J., and Skelton H. G., 1999, British Journal of Dermatology, Vol. 141, No. 3, BritishAssociation of Dermatologists, (558-561). |
| Customized Product Available | No |
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