Plot No. C40, Road No. 21Y, MIDC, Wagle Industrial Estate,
Thane(W)-400604, MH, India.
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Carbon Utilization Test#CC293D
General Information
Yeast Nitrogen Base without Amino Acids is used for investigating carbon and nitrogen requirements of yeasts.
Composition
| Ingredients | Gms / Litre |
|---|---|
| Ammonium sulphate | 5.000 |
| Monopotassium phosphate | 1.000 |
| Magnesium sulphate | 0.500 |
| Sodium chloride | 0.100 |
| Calcium chloride | 0.100 |
| Boric acid | 0.0005 |
| Copper sulphate | 0.00004 |
| Potassium iodide | 0.0001 |
| Ferric chloride | 0.0002 |
| Manganese sulphate | 0.0004 |
| Sodium molybdate | 0.0002 |
| Zinc sulphate | 0.0004 |
| Biotin | 0.000002 |
| Calcium pantothenate | 0.0004 |
| Folic acid | 0.000002 |
| Inositol | 0.002 |
| Niacin | 0.0004 |
| p-Amino benzoic acid (PABA) | 0.0002 |
| Riboflavin (Vitamin B2) | 0.0002 |
| Pyridoxine hydrochloride | 0.0004 |
| Thiamine hydrochloride | 0.0004 |
| Final pH (at 25°C) | 5.4±0.2 |
Formula adjusted, standardized to suit performance parameters
Directions
For best results the medium is prepared in 10X strength. Suspend 6.7 grams in 100 ml distilled water. Add 5 grams dextrose or an equivalent amount of other carbohydrate and other chemicals like amino acids that modify growth of yeasts as desired. Ensure complete solution and sterilize by filtration. For use, dilute 0.5 ml 10X medium to make 5 ml with sterile distilled water. Mix well.
Principle And Interpretation
Yeast Nitrogen Base without Amino Acids is formulated as per Wickerham (1, 2) and is used for investigating amino acid and carbohydrate requirement of yeasts. This medium has the same composition as Yeast Nitrogen Base (M139) medium except the amino acids histidine, methionine and tryptophan.
Inoculate media tubes with very light inoculum and incubate at 25°C for 6-7 days and again for 20-24 days. Draw lines with India ink on a paper. If lines are not seen or appear diffused through the culture, the test is considered positive and if the lines are distinguishable, test is considered negative.
Quality Control
pH: 5.20-5.60
Appearance: White to cream homogeneous free flowing powder
Colour and Clarity: Colourless to light yellow coloured clear solution without any precipitate
Reaction: Reaction of 0.67% w/v aqueous solution at 25°C. pH: 5.4±0.2
Cultural Response
M878: Cultural characteristics observed after an incubation at 25-30°C for 6-7 days (longer if necessary upto 24 days).
| Organism | Growth (Plain) | Growth with dextrose |
|---|---|---|
| Kloeckera apiculata ATCC 9774 | none-poor | good |
| Saccharomyces uvarum ATCC 28098 | none-poor | good |
Storage and Shelf Life
Store dehydrated powder and the prepared medium at 2 - 8°C. Use before expiry date on the label.
| Product Name | Yeast Nitrogen Base w/o Amino Acids |
|---|---|
| SKU | M878 |
| Product Type | Regular |
| Physical Form | Powder |
| Origin | Chemically defined |
| Packaging type | HDPE |
| References | 1. Wickerham L. J., 1951, U.S. Dept. Agric. Tech. Bull. No. 1029. |
| Customized Product Available | No |
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Yeast Nitrogen Base
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